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The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells
https://tokushima-u.repo.nii.ac.jp/records/2008608
https://tokushima-u.repo.nii.ac.jp/records/2008608a100d239-9571-402b-8168-36a647561c6d
| 名前 / ファイル | ライセンス | アクション |
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paejp_468_7_1171.pdf (1.95 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||
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| 公開日 | 2021-04-22 | |||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s00424-016-1806-9 | |||||||||||||||||||||
| 関連名称 | 10.1007/s00424-016-1806-9 | |||||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells | |||||||||||||||||||||
| 著者 |
ハヤシ
× ハヤシ
× 稲垣, 明浩
WEKO
1249
× Novak, I.
× Matsuda, H.
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| 抄録 | ||||||||||||||||||||||
| 内容記述 | Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, adenosine regulates transepithelial anion secretion in duct cells and is considered to play a role in acini-to-duct signaling. To identify the functional adenosine receptors and Cl− channels important for anion secretion, we herein performed experiments on Capan-1, a human pancreatic duct cell line, using open-circuit Ussing chamber and gramicidin-perforated patch-clamp techniques. The luminal addition of adenosine increased the negative transepithelial potential difference (V te) in Capan-1 monolayers with a half-maximal effective concentration value of approximately 10 μM, which corresponded to the value obtained on whole-cell Cl− currents in Capan-1 single cells. The effects of adenosine on V te, an equivalent short-circuit current (I sc), and whole-cell Cl− currents were inhibited by CFTRinh-172, a cystic fibrosis transmembrane conductance regulator (CFTR) Cl− channel inhibitor. The adenosine A2B receptor agonist, BAY 60-6583, increased I sc and whole-cell Cl− currents through CFTR Cl− channels, whereas the A2A receptor agonist, CGS 21680, had negligible effects. The A2B receptor antagonist, PSB 603, inhibited the response of I sc to adenosine. Immunohistochemical analysis showed that the A2A and A2B receptors colocalized with Ezrin in the luminal membranes of Capan-1 monolayers and in rat pancreatic ducts. Adenosine elicited the whole-cell Cl− currents in guinea pig duct cells. These results demonstrate that luminal adenosine regulates anion secretion by activating CFTR Cl− channels via adenosine A2B receptors on the luminal membranes of Capan-1 cells. The present study endorses that purinergic signaling is important in the regulation of pancreatic secretion. | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | Adenosine receptor | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | CFTR | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | Cl− channel | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | Duct | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | Pancreas | |||||||||||||||||||||
| 書誌情報 |
en : Pflügers Archiv: European Journal of Physiology 巻 468, 号 7, p. 1171-1181, 発行日 2016-03-11 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 00316768 | |||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 14322013 | |||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||
| 収録物識別子 | AA00771833 | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||
| 権利情報 | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | |||||||||||||||||||||
| EID | ||||||||||||||||||||||
| 識別子 | 314700 | |||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||
