| Item type |
文献 / Documents(1) |
| 公開日 |
2021-08-26 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1080/14686996.2016.1221726 |
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関連名称 |
10.1080/14686996.2016.1221726 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment |
| 著者 |
Hasan, Mahadi
田良島, 典子
フジカワ, コウキ
オオギタ, タカシ
ハマ, ススム
田中, 保
齋藤, 博幸
南川, 典昭
小暮, 健太朗
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| 抄録 |
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内容記述 |
An intelligent shRNA expression device (iRed) contains the minimum essential components needed for shRNA production in cells, and could be a novel tool to regulate target genes. However, general delivery carriers consisting of cationic polymers/lipids could impede function of a newly generated shRNA via electrostatic interaction in the cytoplasm. Recently, we found that faint electric treatment (fET) of cells enhanced delivery of siRNA and functional nucleic acids into the cytoplasm in the absence of delivery carriers. Here, we examined fET of cells stably expressing luciferase in the presence of iRed encoding anti-luciferase shRNA. Transfection of lipofectamine 2000 (LFN)/iRed lipoplexes showed an RNAi effect, but fET-mediated iRed transfection did not, likely because of the endosomal localization of iRed after delivery. However, fET in the presence of lysosomotropic agent chloroquine significantly improved the RNAi effect of iRed/fET to levels that were higher than those for the LFN/iRed lipoplexes. Furthermore, the amount of lipid droplets in adipocytes significantly decreased following fET with iRed against resistin in the presence of chloroquine. Thus, iRed could be a useful tool to regulate target genes following fET-mediated cytoplasmic delivery with endosomal escape devices. |
| キーワード |
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|
主題 |
iRed |
| キーワード |
|
|
主題 |
4′-thioDNA |
| キーワード |
|
|
主題 |
RNAi effect |
| キーワード |
|
|
主題 |
faint electric treatment |
| キーワード |
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|
主題 |
cytoplasmic delivery |
| 書誌情報 |
en : Science and Technology of Advanced Materials
巻 17,
号 1,
p. 554-562,
発行日 2016-09-16
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
14686996 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
18785514 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11561821 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11539797 |
| 出版者 |
|
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出版者 |
Taylor & Francis |
| 出版者 |
|
|
出版者 |
National Institute for Materials Science |
| 権利情報 |
|
|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| EID |
|
|
識別子 |
317627 |
| 言語 |
|
|
言語 |
eng |
stam_17_1_554.pdf (2.66 MB)
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