| Item type |
文献 / Documents(1) |
| 公開日 |
2021-05-14 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.3390/scipharm84030507 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3390/scipharm84030507 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Anomalous Separation of Small Y-Chromosomal DNA Fragments on Microchip Electrophoresis |
|
言語 |
en |
| 著者 |
Jabasini, Mohammad
エヴィス, アシュラフ
佐藤, 陽一
中堀, 豊
馬場, 嘉信
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We investigated an anomalous DNA separation where two DNA fragments from the human Y-chromosome sY638 (64 bp) and sY592 (65 bp), with only one base pair difference, were separated. This result is abnormal since in a previous study, we found that 5 bp was the minimum difference between two DNA fragments that the microchip electrophoresis system can separate. The formation of a mini-loop in the structure of the DNA fragment of sY638 (64 bp) was strongly expected to be the reason. To investigate this, we synthesized three modified DNA fragments for sY638 (64 bp), and the modifications were in two expected locations for possible mini-loop formation. Later, the separation between sY592 (65 bp) and the three modified fragments of sY638 (64 bp) was not possible. Thus, we conclude that the formation of a mini-loop in the structure of the DNA is the reason behind this anomalous separation. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
anomalous separation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microchip electrophoresis |
| 書誌情報 |
en : Scientia Pharmaceutica
巻 84,
号 3,
p. 507-513,
発行日 2016-05-26
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
22180532 |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
| EID |
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|
識別子 |
313257 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
scipharm_84_3_507.pdf (802 KB)
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