| Item type |
文献 / Documents(1) |
| 公開日 |
2022-07-05 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1093/femsle/fnx258 |
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言語 |
ja |
|
|
関連名称 |
10.1093/femsle/fnx258 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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|
タイトル |
Rapid screening method for detecting highly pathogenic Streptococcus intermedius strains carrying a mutation in the lacR gene |
|
言語 |
en |
| 著者 |
友安, 俊文
マトバ, マサキ
タカオ, アユコ
田端, 厚之
Whiley, Robert A.
マエダ, ノブコ
長宗, 秀明
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| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Streptococcus intermedius is a member of the normal human commensal flora and secretes a human-specific cytolysin intermedilysin (ILY) as a major virulence factor. Expression of ily is repressed by LacR and loss-of-function mutations of LacR are observed in many ILY high-producing strains isolated from deep-seated abscesses, suggesting that high ILY production is necessary for increased virulence. However, because ILY exhibits no β-hemolysis on animal blood agar plates, differentiating ILY high- and low-producing strains using conventional laboratory methods is not possible. Interestingly, S. intermedius also produces glycosidases, including MsgA and NanA, which exhibit N-acetyl-β-D-glucosaminidase and neuraminidase activities, respectively. Moreover, MsgA expression, but not NanA, is negatively regulated by LacR. Here we measured the activities of MsgA, NanA and ILY in strains isolated from clinical specimens and dental plaque to determine the correlation between these glycosidase activities and ILY hemolytic activity. Hemolytic activity showed a strong positive correlation with MsgA and a weak negative correlation with NanA activities. Therefore, we calculated the ratio of MsgA and NanA activity (M/N ratio). This value showed a stronger positive correlation (r = 0.81) with ILY hemolytic activity and many strains with high M/N ratios (> 2) were ILY high-producers with loss-of-function mutations in LacR. |
|
言語 |
en |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Rapid screening method for highly pathogenic S. intermedius strains based on the ratio of activities of MsgA and NanA resulting from lacR mutations. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Streptococcus intermedius |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
intermedilysin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
screening |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
MsgA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
NanA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
LacR |
| 書誌情報 |
en : FEMS Microbiology Letters
巻 365,
号 3,
p. fnx258,
発行日 2017-12-06
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15746968 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03781097 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00193861 |
| 出版者 |
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|
出版者 |
Federation of European Microbiological Societies |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Oxford University Press |
|
言語 |
en |
| 備考 |
|
|
言語 |
ja |
|
値 |
This is a pre-copyedited, author-produced version of an article accepted for publication in FEMS Microbiology Letters following peer review. The version of record Toshifumi Tomoyasu, Masaki Matoba, Ayuko Takao, Atsushi Tabata, Robert A Whiley, Nobuko Maeda, Hideaki Nagamune, Rapid screening method for detecting highly pathogenic Streptococcus intermedius strains carrying a mutation in the lacR gene, FEMS Microbiology Letters, Volume 365, Issue 3, February 2018, fnx258 is available online at: https://doi.org/10.1093/femsle/fnx258 |
| EID |
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|
識別子 |
340526 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
femsle_365_3_fnx258.pdf (954 KB)
