| Item type |
文献 / Documents(1) |
| 公開日 |
2021-03-12 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1242/dev.122598 |
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関連名称 |
10.1242/dev.122598 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus |
| 著者 |
濱田, 良真
バンドウ, テツヤ
ナカムラ, タロウ
石丸, 善康
三戸, 太郎
野地, 澄晴
トミオカ, ケンジ
大内, 淑代
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| 抄録 |
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内容記述 |
Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb'E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb'Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb'E(z)RNAi and was increased by Gb'UtxRNAi. Regenerated Gb'E(z)RNAi cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb'UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the Gb'E(z)RNAi regenerating leg, the Gb'dac expression domain expanded in the tarsus. By contrast, in the Gb'UtxRNAi regenerating leg, Gb'Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression. |
| キーワード |
|
|
主題 |
Regeneration |
| キーワード |
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|
主題 |
Epigenetics |
| キーワード |
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|
主題 |
Histone H3K27 |
| キーワード |
|
|
主題 |
Gryllus bimaculatus |
| キーワード |
|
|
主題 |
Polycomb |
| 書誌情報 |
en : Development
巻 142,
号 17,
p. 2916-2927,
発行日 2015-09-01
|
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09501991 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14779129 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10667805 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12024599 |
| 出版者 |
|
|
出版者 |
The Company of Biologists |
| EID |
|
|
識別子 |
303095 |
| 言語 |
|
|
言語 |
eng |
dev_142_17_2916.pdf (4.54 MB)
