| Item type |
文献 / Documents(1) |
| 公開日 |
2021-06-04 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.3390/antiox9121193 |
|
|
関連名称 |
10.3390/antiox9121193 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Identification of Dietary Phytochemicals Capable of Enhancing the Autophagy Flux in HeLa and Caco-2 Human Cell Lines |
| 著者 |
大西, 康太
ヤノ, サトシ
フジモト, モエ
サカイ, マイコ
ハルモト, エリカ
フルイチ, アイリ
増田, 真志
大南, 博和
(山中)奥村, 仙示
ハラ, タイチ
竹谷, 豊
|
| 抄録 |
|
|
内容記述 |
Autophagy is a major degradation system for intracellular macromolecules. Its decline with age or obesity is related to the onset and development of various intractable diseases. Although dietary phytochemicals are expected to enhance autophagy for preventive medicine, few studies have addressed their effects on the autophagy flux, which is the focus of the current study. Herein, 67 dietary phytochemicals were screened using a green fluorescent protein (GFP)-microtubule-associated protein light chain 3 (LC3)-red fluorescent protein (RFP)-LC3ΔG probe for the quantitative assessment of autophagic degradation. Among them, isorhamnetin, chrysoeriol, 2,2′,4′-trihydroxychalcone, and zerumbone enhanced the autophagy flux in HeLa cells. Meanwhile, analysis of the structure–activity relationships indicated that the 3′-methoxy-4′-hydroxy group on the B-ring in the flavone skeleton and an ortho-phenolic group on the chalcone B-ring were crucial for phytochemicals activities. These active compounds were also effective in colon carcinoma Caco-2 cells, and some of them increased the expression of p62 protein, a typical substrate of autophagic proteolysis, indicating that phytochemicals impact p62 levels in autophagy-dependent and/or -independent manners. In addition, these compounds were characterized by distinct modes of action. While isorhamnetin and chrysoeriol enhanced autophagy in an mTOR signaling-dependent manner, the actions of 2,2′,4′-trihydroxychalcone and zerumbone were independent of mTOR signaling. Hence, these dietary phytochemicals may prove effective as potential preventive or therapeutic strategies for lifestyle-related diseases. |
| キーワード |
|
|
主題 |
phytochemicals |
| キーワード |
|
|
主題 |
autophagy flux |
| キーワード |
|
|
主題 |
structure–activity relationship |
| キーワード |
|
|
主題 |
mTOR |
| キーワード |
|
|
主題 |
p62 |
| 書誌情報 |
en : Antioxidants
巻 9,
号 12,
p. 1193,
発行日 2020-11-27
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20763921 |
| 出版者 |
|
|
出版者 |
MDPI |
| 権利情報 |
|
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
372641 |
| 言語 |
|
|
言語 |
eng |
antiox_9_12_1193.pdf (2.73 MB)
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