| Item type |
文献 / Documents(1) |
| 公開日 |
2021-07-26 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.7554/eLife.54983 |
|
|
関連名称 |
10.7554/eLife.54983 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
AirID, a novel proximity biotinylation enzyme, for analysis of protein–protein interactions |
| 著者 |
キド, コウキ
ヤマナカ, サトシ
ナカノ, ショウゴ
茂谷, 康
シノハラ, ソウタ
ノザワ, アキラ
小迫, 英尊
イトウ, ソウヘイ
サワサキ, タツヤ
|
| 抄録 |
|
|
内容記述 |
Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism. However, there have been some improvements in the enzymes that are used for that purpose. Here, we demonstrate a novel BirA enzyme, AirID (ancestral BirA for proximity-dependent biotin identification), which was designed de novo using an ancestral enzyme reconstruction algorithm and metagenome data. AirID-fusion proteins such as AirID-p53 or AirID-IκBα indicated biotinylation of MDM2 or RelA, respectively, in vitro and in cells, respectively. AirID-CRBN showed the pomalidomide-dependent biotinylation of IKZF1 and SALL4 in vitro. AirID-CRBN biotinylated the endogenous CUL4 and RBX1 in the CRL4CRBN complex based on the streptavidin pull-down assay. LC-MS/MS analysis of cells that were stably expressing AirID-IκBα showed top-level biotinylation of RelA proteins. These results indicate that AirID is a novel enzyme for analyzing protein–protein interactions. |
| 書誌情報 |
en : eLife
巻 9,
p. e54983,
発行日 2020-05-11
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2050084X |
| 出版者 |
|
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出版者 |
eLife Sciences Publications |
| 権利情報 |
|
|
権利情報 |
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
| EID |
|
|
識別子 |
366537 |
| 言語 |
|
|
言語 |
eng |
elife_9_e54983.pdf (4.21 MB)
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