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BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING)
https://tokushima-u.repo.nii.ac.jp/records/2008852
https://tokushima-u.repo.nii.ac.jp/records/20088526ddc5453-075f-4dea-8252-ccc94104fa70
| 名前 / ファイル | ライセンス | アクション |
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jbc_295_32_11174.pdf (2.12 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||
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| 公開日 | 2021-08-20 | |||||||||||||||||
| アクセス権 | ||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 出版社版DOI | ||||||||||||||||||
| 関連識別子 | https://doi.org/10.1074/jbc.ra120.014323 | |||||||||||||||||
| 関連名称 | 10.1074/jbc.ra120.014323 | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING) | |||||||||||||||||
| タイトル別表記 | ||||||||||||||||||
| その他のタイトル | BioID using Tamavidin 2-REV | |||||||||||||||||
| 著者 |
茂谷, 康
× 茂谷, 康
WEKO
684
× 小迫, 英尊
WEKO
301
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| 抄録 | ||||||||||||||||||
| 内容記述 | Stimulator of interferon genes (STING) mediates cytosolic DNA-induced innate immune signaling via membrane trafficking. The global identification of proteins that spatiotemporally interact with STING will provide a better understanding of its trafficking mechanisms and of STING signaling pathways. Proximity-dependent biotin identification (BioID) is a powerful technology to identify physiologically relevant protein-protein interactions in living cells. However, biotinylated peptides are rarely detected in the conventional BioID method, which uses streptavidin beads to pull down biotinylated proteins, because the biotin-streptavidin interaction is too strong. As a result, only nonbiotinylated peptides are identified, which cannot be distinguished from peptides of nonspecifically pull-downed proteins. Here, we developed a simple method to efficiently and specifically enrich biotinylated peptides using Tamavidin 2-REV, an engineered avidin-like protein with reversible biotin-binding capability. Using RAW264.7 macrophages stably expressing TurboID-fused STING, we identified and quantified >4,000 biotinylated peptides of STING-proximal proteins. Various endoplasmic reticulum-associated proteins were biotinylated in unstimulated cells, and STING activation caused biotinylation of many proteins located in the Golgi and endosomes. These proteins included those known to interact with activated STING, such as TANK-binding kinase 1 (TBK1), several palmitoyl transferases, and p62/sequestosome 1 (SQSTM1). Furthermore, interferon-induced transmembrane protein 3 (IFITM3), an endolysosome-localized antiviral protein, bound to STING at the late activation stage. These dynamic interaction profiles will provide detailed insights into STING signaling; we propose that our approach using Tamavidin 2-REV would be useful for BioID-based and other biotinylation-based peptide identification methods. | |||||||||||||||||
| 書誌情報 |
en : Journal of Biological Chemistry 巻 295, 号 32, p. 11174-11183, 発行日 2020-06-17 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 00219258 | |||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AA1202441X | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | American Society for Biochemistry and Molecular Biology | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||||
| 権利情報 | ||||||||||||||||||
| 権利情報 | This is an Open Access article under the CC BY license. | |||||||||||||||||
| EID | ||||||||||||||||||
| 識別子 | 366538 | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
