| Item type |
文献 / Documents(1) |
| 公開日 |
2021-06-01 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1128/JVI.00716-16 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1128/JVI.00716-16 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Existence of Two Distinct Infectious Endogenous Retroviruses in Domestic Cats and Their Different Strategies for Adaptation to Transcriptional Regulation |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Transcriptional Regulation of Infectious Feline ERVs |
|
言語 |
en |
| 著者 |
クセ, キョウヘイ
イトウ, ジュンペイ
三宅, 在子
カワサキ, ジュンナ
ワタナベ, シンヤ
Makundi, Isaac
Ngo, Minh Ha
音井, 威重
ニシガキ, カズオ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Endogenous retroviruses (ERVs) are the remnants of ancient retroviral infections of germ cells. Previous work identified one of the youngest feline ERV groups, ERV-DC, and reported that two ERV-DC loci, ERV-DC10 and ERV-DC18 (ERV-DC10/DC18), can replicate in cultured cells. Here, we identified another replication-competent provirus, ERV-DC14, on chromosome C1q32. ERV-DC14 differs from ERV-DC10/DC18 in its phylogeny, receptor usage, and, most notably, transcriptional activities; although ERV-DC14 can replicate in cultured cells, it cannot establish a persistent infection owing to its low transcriptional activity. Furthermore, we examined ERV-DC transcription and its regulation in feline tissues. Quantitative reverse transcription-PCR (RT-PCR) detected extremely low ERV-DC10 expression levels in feline tissues, and bisulfite sequencing showed that 5′ long terminal repeats (LTRs) of ERV-DC10/DC18 are significantly hypermethylated in feline blood cells. Reporter assays found that the 5′-LTR promoter activities of ERV-DC10/DC18 are high, whereas that of ERV-DC14 is low. This difference in promoter activity is due to a single substitution from A to T in the LTR, and reverse mutation at this nucleotide in ERV-DC14 enhanced its replication and enabled it to persistently infect cultured cells. Therefore, ERV-DC LTRs can be divided into two types based on this nucleotide, the A type or T type, which have strong or attenuated promoter activity, respectively. Notably, ERV-DCs with T-type LTRs, such as ERV-DC14, have expanded in the cat genome significantly more than A-type ERV-DCs, despite their low promoter activities. Our results provide insights into how the host controls potentially infectious ERVs and, conversely, how ERVs adapt to and invade the host genome. |
|
言語 |
en |
| 書誌情報 |
en : Journal of Virology
巻 90,
号 20,
p. 9029-9045,
発行日 2016-09-29
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
10985514 |
| 出版者 |
|
|
出版者 |
American Society for Microbiology |
|
言語 |
en |
| EID |
|
|
識別子 |
317350 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
JVI_90_20_9029.pdf (5.46 MB)
