| Item type |
文献 / Documents(1) |
| 公開日 |
2021-07-09 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1111/rda.13005 |
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関連名称 |
10.1111/rda.13005 |
| 出版タイプ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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|
タイトル |
Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes |
| タイトル別表記 |
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その他のタイトル |
Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilisation, and developmental competence of porcine oocytes |
| タイトル別表記 |
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|
その他のタイトル |
Effects of chlorogenic acid on porcine oocytes |
| 著者 |
Nguyen, Thanh-Van
谷原, 史倫
Do, Lanh Thi Kim
サトウ, ヨウコ
タニグチ, マサヤス
タカギ, ミツヒロ
Nguyen, Thanh Van
音井, 威重
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| 抄録 |
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内容記述 |
Chlorogenic acid (CGA) is a quinic acid conjugate of caffeic acid, and a phytochemical found in many fruits and beverages that acts as an antioxidant. The present study investigated the effects of CGA supplementation during in vitro maturation, on in vitro development of porcine oocytes, in order to improve the porcine in vitro production (IVP) system. Oocytes were matured either without (control) or with CGA (10, 50, 100, and 200 µM). Subsequently, the matured oocytes were fertilised, and cultured in vitro for 7 d. The rates of maturation, fertilisation, and blastocyst formation of oocytes matured with 50 µM CGA was significantly (p < 0.05) higher than those of the control oocytes. Hydrogen peroxide (H2O2) is one of the reactive oxygen species and induces DNA damage in porcine oocytes. When oocytes were matured with 1 mM H2O2 to assess the protective effect of CGA, 50 µM CGA supplementation improved the maturation rate and the proportion of DNA-fragmented nuclei in oocytes compared with control oocytes matured without CGA. Moreover, when oocytes were matured with either 50 µM CGA (control) or caffeic acid (10, 50, and 100 µM), the rates of maturation, fertilisation, and the blastocyst formation of oocytes matured with 50 µM CGA were similar to those of oocytes matured with 10 and 50 µM caffeic acid. Our results suggest that CGA has comparable effects to caffeic acid, and in vitro maturation with 50 µM CGA is particularly beneficial to in vitro production of porcine embryos and protects oocytes from DNA damage induced by oxidative stress. Supplementation of CGA to the maturation medium has a potential to improve porcine IVP system. |
| キーワード |
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|
主題 |
antioxidative stress |
| キーワード |
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主題 |
embryo |
| キーワード |
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|
主題 |
DNA fragmentation |
| キーワード |
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|
主題 |
chlorogenic acid |
| キーワード |
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|
主題 |
developmental competence |
| 書誌情報 |
en : Reproduction in Domestic Animals
巻 52,
号 6,
p. 969-975,
発行日 2017-06-28
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
14390531 |
| 出版者 |
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出版者 |
Wiley-VCH |
| 備考 |
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|
値 |
This is the peer reviewed version of the following article: Nguyen, T-V, Tanihara, F, Do, LTK et al. Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes. Reprod Dom Anim. 2017; 52: 969– 975., which has been published in final form at https://doi.org/10.1111/rda.13005. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| EID |
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|
識別子 |
346495 |
| 言語 |
|
|
言語 |
eng |
rda_52_6_969.pdf (416 KB)
