| Item type |
文献 / Documents(1) |
| 公開日 |
2021-07-09 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1111/asj.13049 |
|
|
関連名称 |
10.1111/asj.13049 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization |
| タイトル別表記 |
|
|
その他のタイトル |
EFFECT OF CGA ON ELECTROPLATED EMBRYOS |
| 著者 |
Nguyen, Thanh-Van
Wittayarat, Manita
Do, Lanh Thi Kim
Nguyen, Thanh Van
ニイ, マサヒロ
Namula, Zhao
クニハラ, トシキ
谷原, 史倫
平田, 真樹
音井, 威重
|
| 抄録 |
|
|
内容記述 |
Electroporation is the technique of choice to introduce an exogenous gene into embryos for transgenic animal production. Although this technique is practical and effective, embryonic damage caused by electroporation treatment remains a major problem. This study was conducted to evaluate the optimal culture system for electroporation-treated porcine embryos by supplementation of chlorogenic acid (CGA), a potent antioxidant, during in vitro oocyte maturation. The oocytes were treated with various concentrations of CGA (0, 10, 50, and 100 µM/L) through the duration of maturation for 44 h. The treated oocytes were then fertilized, electroporated at 30 V/mm with five 1-msec unipolar pulses, and subsequently cultured in vitro until development into the blastocyst stage. Without electroporation, the treatment with 50 µM/L CGA had useful effects on the maturation rate of oocytes, the total cell number, and the apoptotic nucleus indices of blastocysts. When the oocytes were electroporated after in vitro fertilization, the treatment with 50 μM CGA supplementation significantly improved the rate of oocytes that developed into blastocysts and reduced the apoptotic nucleus indices (4.7% and 7.6, respectively) compared with those of the untreated group (1.4% and 13.0, respectively). These results suggested that supplementation with 50 μM CGA during maturation improves porcine embryonic development and quality of electroporation-treated embryos. |
| キーワード |
|
|
主題 |
antioxidant |
| キーワード |
|
|
主題 |
chlorogenic acid |
| キーワード |
|
|
主題 |
electroporation |
| キーワード |
|
|
主題 |
embryo |
| キーワード |
|
|
主題 |
oxidative stress |
| 書誌情報 |
en : Animal Science Journal
巻 89,
号 8,
p. 1207-1213,
発行日 2018-05-27
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
17400929 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11677214 |
| 出版者 |
|
|
出版者 |
Japanese Society of Animal Science |
| 出版者 |
|
|
出版者 |
Wiley |
| 備考 |
|
|
値 |
This is the peer reviewed version of the following article: Nguyen, T-V, Wittayarat, M, Do, LTK, et al. Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization. Anim Sci J. 2018; 89: 1207– 1213., which has been published in final form at https://doi.org/10.1111/asj.13049. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| EID |
|
|
識別子 |
346499 |
| 言語 |
|
|
言語 |
eng |
asj_89_8_1207.pdf (397 KB)
