| Item type |
文献 / Documents(1) |
| 公開日 |
2021-08-06 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1016/j.ijpharm.2021.120966 |
|
|
関連名称 |
10.1016/j.ijpharm.2021.120966 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
A simple, fast, and orientation-controllable technology for preparing antibody-modified liposomes |
| 著者 |
ヒラタ, ユウマ
タシマ, リホ
ミツハシ, ナオト
米田, 晋太朗
大園, 瑞音
福田, 達也
マジマ, エイジ
小暮, 健太朗
|
| 抄録 |
|
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内容記述 |
Modification with antibodies is a useful strategy for the delivery of nanoparticles to target cells. However, the complexity of the required chemical modifications makes them time-consuming and low efficiency, and the orientation of the antibody is challenging to control. To develop a simple, fast, effective, and orientation-controllable technology, we employed staphylococcal protein A, which can bind to the Fc region of antibodies, as a tool for conjugating antibodies to nanoparticles. Specifically, we modified the C-domain dimer of protein A to contain a lysine cluster to create a molecule, DPACK, that would electrostatically bind to anionic liposomes. Using this protein, antibody-modified liposomes can be prepared in 35 minutes with two steps: (1) interaction of DPACK with liposomes and (2) interaction of an antibody with DPACK-modified liposomes. Binding efficiencies of DPACK with liposomes and IgG with DPACK-modified liposomes were 75% and 72-84%, respectively. Uptake of liposomes modified with anti-epidermal growth factor receptor (EGFR) antibodies via DPACK by EGFR-expressing cancer cells was significantly higher than that of unmodified liposomes, and the liposomes accumulated in tumors and colocalized with EGFR. This simple, fast, effective and orientation-controllable technology for preparing antibody-modified liposomes will be useful for active targeting drug delivery. |
| キーワード |
|
|
主題 |
Antibody-modified liposomes |
| キーワード |
|
|
主題 |
modified protein A |
| キーワード |
|
|
主題 |
active targeting |
| 書誌情報 |
en : International Journal of Pharmaceutics
巻 607,
p. 120966,
発行日 2021-08-02
|
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03785173 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00680771 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11530828 |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
377807 |
| 言語 |
|
|
言語 |
eng |
ijpharm_607_120966.pdf (1.66 MB)
