| Item type |
文献 / Documents(1) |
| 公開日 |
2021-08-04 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1093/bbb/zbab078 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1093/bbb/zbab078 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Characterization of a novel moderate-substrate specificity amino acid racemase from the hyperthermophilic archaeon Thermococcus litoralis |
|
言語 |
en |
| 著者 |
川上, 竜巳
キノシタ, チナツ
カワセ, トモキ
サトウ, ミキオ
林, 順司
櫻庭, 春彦
大島, 敏久
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The amino acid sequence of the OCC_10945 gene product from the hyperthermophilic archaeon Thermococcus litoralis DSM5473, originally annotated as γ-aminobutyrate aminotransferase, is highly similar to that of the uncharacterized pyridoxal 5ʹ-phosphate (PLP)-dependent amino acid racemase from Pyrococcus horikoshii. The OCC_10945 enzyme was successfully overexpressed in Escherichia coli by co-expression with a chaperone protein. The purified enzyme demonstrated PLP-dependent amino acid racemase activity primarily toward Met and Leu. Although PLP contributed to enzyme stability, it only loosely bound to this enzyme. Enzyme activity was strongly inhibited by several metal ions, including Co2+ and Zn2+, and non-substrate amino acids such as L-Arg and L-Lys. These results suggest that the underlying PLP-binding and substrate recognition mechanisms in this enzyme are significantly different from those of the other archaeal and bacterial amino acid racemases. This is the first description of a novel PLP-dependent amino acid racemase with moderate substrate specificity in hyperthermophilic archaea. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
pyridoxal 5ʹ-phosphate |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
amino acid racemase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
substrate specificity |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Thermococcus litoralis |
| 書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry
巻 85,
号 7,
p. 1650-1657,
発行日 2021-05-04
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09168451 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13476947 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10824164 |
| 出版者 |
|
|
出版者 |
Japan Society for Bioscience, Biotechnology, and Agrochemistry |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Oxford University Press |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience, Biotechnology, and Biochemistry following peer review. The version of record Ryushi Kawakami, Chinatsu Kinoshita, Tomoki Kawase, Mikio Sato, Junji Hayashi, Haruhiko Sakuraba, Toshihisa Ohshima, Characterization of a novel moderate-substrate specificity amino acid racemase from the hyperthermophilic archaeon Thermococcus litoralis, Bioscience, Biotechnology, and Biochemistry, Volume 85, Issue 7, July 2021, Pages 1650–1657 is available online at: https://doi.org/10.1093/bbb/zbab078. |
| EID |
|
|
識別子 |
377905 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
bbb_85_7_1650.pdf (765 KB)
