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Neurite Outgrowth-Promoting Activity of Compounds in PC12 Cells from Sunflower Seeds
https://tokushima-u.repo.nii.ac.jp/records/2009163
https://tokushima-u.repo.nii.ac.jp/records/20091632ab3c531-5b9c-4489-9025-bf2b8e3e4fae
| 名前 / ファイル | ライセンス | アクション |
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molecules_25_20_4748.pdf (2.34 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2021-09-06 | |||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.3390/molecules25204748 | |||||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||||||
| 関連名称 | 10.3390/molecules25204748 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Neurite Outgrowth-Promoting Activity of Compounds in PC12 Cells from Sunflower Seeds | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 著者 |
古賀, 武尊
× 古賀, 武尊× 阪本, 鷹行
WEKO
760
× 櫻谷, 英治
WEKO
716
× 田井, 章博
WEKO
959
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| 内容記述タイプ | Abstract | |||||||||||||||||||||||
| 内容記述 | In the current super-aging society, the establishment of methods for prevention and treatment of Alzheimer’s disease (AD) is an urgent task. One of the causes of AD is thought to be a decrease in the revel of nerve growth factor (NGF) in the brain. Compounds showing NGF-mimicking activity and NGF-enhancing activity have been examined as possible agents for improving symptoms. In the present study, sunflower seed extract was found to have neurite outgrowth-promoting activity, which is an NGF-enhancing activity, in PC12 cells. To investigate neurite outgrowth-promoting compounds from sunflower seed extract, bioassay-guided purification was carried out. The purified active fraction was obtained by liquid-liquid partition followed by some column chromatographies. Proton nuclear magnetic resonance and gas chromatography-mass spectrometry analyses of the purified active fraction indicated that the fraction was a mixture of β-sitosterol, stigmasterol and campesterol, with β-sitosterol being the main component. Neurite outgrowth-promoting activities of β-sitosterol, stigmasterol, campesterol and cholesterol were evaluated in PC12 cells. β-Sitosterol and stigmasterol showed the strongest activity of the four sterol compounds (β-sitosterol ≈ stigmasterol > campesterol > cholesterol), and cholesterol did not show any activity. The results indicated that β-sitosterol was the major component responsible for the neurite outgrowth-promoting activity of sunflower seeds. Results of immunostaining also showed that promotion by β-sitosterol of neurite formation induced by NGF was accompanied by neurofilament expression. β-Sitosterol, which showed NGF-enhancing activity, might be a candidate ingredient in food for prevention of AD. | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Alzheimer’s disease | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | sunflower seed | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | NGF | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | β-sitosterol | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | neurite outgrowth-promoting activity | |||||||||||||||||||||||
| 書誌情報 |
en : Molecules 巻 25, 号 20, p. 4748, 発行日 2020-10-16 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 14203049 | |||||||||||||||||||||||
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| 出版者 | MDPI | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||||||||||||||||||||
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| 識別子 | 371840 | |||||||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||
