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Simple method for large-scale production of macrophage activating factor GcMAF
https://tokushima-u.repo.nii.ac.jp/records/2009174
https://tokushima-u.repo.nii.ac.jp/records/2009174bd3d46c0-e8f4-41d6-a443-4ba041a8ed79
| 名前 / ファイル | ライセンス | アクション |
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srep_10_19122.pdf (2.87 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||||||||||||||
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| 公開日 | 2021-09-07 | |||||||||||||||||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1038/s41598-020-75571-y | |||||||||||||||||||||||||||||||||||||||||
| 関連名称 | 10.1038/s41598-020-75571-y | |||||||||||||||||||||||||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||||||||||||||
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| タイトル | Simple method for large-scale production of macrophage activating factor GcMAF | |||||||||||||||||||||||||||||||||||||||||
| 著者 |
ナベシマ, ヨウコ
× ナベシマ, ヨウコ
× アベ, チアキ
× カワウチ, タケシ
× ヒロイ, トモコ
× 宇都, 義浩
WEKO
116
× ナベシマ, ヨウイチ
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| 内容記述 | Human group-specific component protein (Gc protein) is a multifunctional serum protein which has three common allelic variants, Gc1F, Gc1S and Gc2 in humans. Gc1 contains an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine (GalNAc)] on the threonine420 (Thr420) residue and can be converted to a potent macrophage activating factor (GcMAF) by selective removal of sialic acid and galactose, leaving GalNAc at Thr420. In contrast, Gc2 is not glycosylated. GcMAF is considered a promising candidate for immunotherapy and antiangiogenic therapy of cancers and has attracted great interest, but it remains difficult to compare findings among research groups because different procedures have been used to prepare GcMAF. Here, we present a simple, practical method to prepare high-quality GcMAF by overexpressing Gc-protein in a serum-free suspension culture of ExpiCHO-S cells, without the need for a de-glycosylation step. We believe this protocol is suitable for large-scale production of GcMAF for functional analysis and clinical testing. | |||||||||||||||||||||||||||||||||||||||||
| 書誌情報 |
en : Scientific Reports 巻 10, p. 19122, 発行日 2020-11-05 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||||||||||||||
| 収録物識別子 | 20452322 | |||||||||||||||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||||||||||||||
| 権利情報 | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | |||||||||||||||||||||||||||||||||||||||||
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| 識別子 | 373314 | |||||||||||||||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||||||||||||||
