| Item type |
文献 / Documents(1) |
| 公開日 |
2021-09-13 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1038/s42003-021-01739-5 |
|
|
関連名称 |
10.1038/s42003-021-01739-5 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Structure–function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis |
| 著者 |
カジウラ, ヒロユキ
ヨシザワ, タクヤ
トクモト, ユウジ
スズキ, ノブアキ
タケノ, シンヤ
タケノ
ヤマシタ, タクヤ
タナカ, シュンイチ
カネコ, ヨシノブ
フジヤマ, カズヒト
マツムラ, ヒロヨシ
中澤, 慶久
|
| 抄録 |
|
|
内容記述 |
Some plant trans-1,4-prenyltransferases (TPTs) produce ultrahigh molecular weight trans-1,4-polyisoprene (TPI) with a molecular weight of over 1.0 million. Although plant-derived TPI has been utilized in various industries, its biosynthesis and physiological function(s) are unclear. Here, we identified three novel Eucommia ulmoides TPT isoforms—EuTPT1, 3, and 5, which synthesized TPI in vitro without other components. Crystal structure analysis of EuTPT3 revealed a dimeric architecture with a central hydrophobic tunnel. Mutation of Cys94 and Ala95 on the central hydrophobic tunnel no longer synthesizd TPI, indicating that Cys94 and Ala95 were essential for forming the dimeric architecture of ultralong-chain TPTs and TPI biosynthesis. A spatiotemporal analysis of the physiological function of TPI in E. ulmoides suggested that it is involved in seed development and maturation. Thus, our analysis provides functional and mechanistic insights into TPI biosynthesis and uncovers biological roles of TPI in plants. |
| 書誌情報 |
en : Communications Biology
巻 4,
p. 215,
発行日 2021-02-16
|
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
23993642 |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 権利情報 |
|
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
|
|
識別子 |
381975 |
| 言語 |
|
|
言語 |
eng |
cb_4_215.pdf (3.05 MB)
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