| Item type |
文献 / Documents(1) |
| 公開日 |
2021-09-28 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1002/eji.202048764 |
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関連名称 |
10.1002/eji.202048764 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
A novel method to identify Post-Aire stages of medullary thymic epithelial cell differentiation |
| タイトル別表記 |
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その他のタイトル |
Leukocyte and lymphoid organ ontogeny |
| 著者 |
Ferreirinha, Pedro
Ribeiro, Camila
森本, 純子
Landry, Jonathan J. M.
松本, 穣
Meireles, Catarina
White, Andrea J.
大東, いずみ
Araújo, Leonor
Benes, Vladimir
高浜, 洋介
Anderson, Graham
松本, 満
Alves, Nuno L.
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| 抄録 |
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内容記述 |
Autoimmune regulator+ (Aire) medullary thymic epithelial cells (mTECs) play a critical role in tolerance induction. Several studies demonstrated that Aire+mTECs differentiate further into Post-Aire cells. Yet, the identification of terminal stages of mTEC maturation depends on unique fate-mapping mouse models. Herein, we resolve this limitation by segmenting the mTEChi(MHCIIhiCD80hi) compartment into mTECA/hi (CD24−Sca1−), mTECB/hi (CD24+Sca1−), and mTECC/hi (CD24+Sca1+). While mTECA/hi included mostly Aire-expressing cells, mTECB/hi contained Aire+ and Aire− cells and mTECC/hi were mainly composed of cells lacking Aire. The differential expression pattern of Aire led us to investigate the precursor-product relationship between these subsets. Strikingly, transcriptomic analysis of mTECA/hi, mTECB/hi, and mTECC/hi sequentially mirrored the specific genetic program of Early-, Late- and Post-Aire mTECs. Corroborating their Post-Aire nature, mTECC/hi downregulated the expression of tissue-restricted antigens, acquired traits of differentiated keratinocytes, and were absent in Aire-deficient mice. Collectively, our findings reveal a new and simple blueprint to survey late stages of mTEC differentiation. |
| キーワード |
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|
主題 |
autoimmune regulator |
| キーワード |
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|
主題 |
differentiation |
| キーワード |
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|
主題 |
medullary thymic epithelial cell |
| キーワード |
|
|
主題 |
thymus |
| キーワード |
|
|
主題 |
tolerance induction |
| 書誌情報 |
en : European Journal of Immunology
巻 51,
号 2,
p. 311-318,
発行日 2020-08-26
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
15214141 |
| 出版者 |
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出版者 |
Wiley |
| 出版者 |
|
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出版者 |
European Federation of Immunological Societies |
| 権利情報 |
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|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
| EID |
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|
識別子 |
374058 |
| 言語 |
|
|
言語 |
eng |
eji_51_2_311.pdf (1.86 MB)
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