| Item type |
文献 / Documents(1) |
| 公開日 |
2021-12-02 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.5194/aab-64-265-2021 |
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関連名称 |
10.5194/aab-64-265-2021 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs |
| タイトル別表記 |
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その他のタイトル |
Improvement of the in vitro fertilization and embryo development of microminipigs |
| 著者 |
Namula, Zhao
イスミ, ヤスヒロ
サトウ, ヨウコ
Le, Quynh Anh
Lin, Qingyi
タケバヤシ, コウキ
平田, 真樹
谷原, 史倫
ソンキテイデイロ, チヨンマナート
| ja |
ソンキテイデイロ, チヨンマナート
|
| ja-Kana |
トンキッチィディロク, チヨンマナート
|
| en |
Thongkittidilok, Chommanart
|
Search repository
音井, 威重
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| 抄録 |
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内容記述 |
This study aimed to compare the quality and the penetration ability of frozen–thawed spermatozoa from three microminipigs and Large White boars and to evaluate the effects of caffeine and heparin as well as the sperm–oocyte co-incubation length on the fertilization and embryonic development in vitro. Results showed that the fertilization rates of spermatozoa from three microminipig boars were significantly lower than those of a Large White boar. In the post-thaw spermatozoa from one of three microminipig boars, the sperm quality, penetration ability, and the oocyte development after in vitro fertilization were significantly lower than those of the spermatozoa from other boars. The caffeine supplementation in the fertilization media increased the rates of fertilization and blastocyst formation for the microminipig spermatozoa with low sperm quality. In addition to caffeine supplementation, the rates of fertilization and blastocyst formation after using microminipig spermatozoa were significantly higher with a 10 h sperm–oocyte co-incubation than with 3 h of co-incubation length. Our results indicate that the differences between the males and the breed influence the quality and fertility of frozen–thawed boar spermatozoa. In conclusion, the presence of caffeine in the in vitro fertilization (IVF) medium and adequate length of sperm–oocyte co-incubation may have beneficial effects for improving IVF results when using microminipig spermatozoa with low quality. |
| 書誌情報 |
en : Archives Animal Breeding
巻 64,
号 1,
p. 265-271,
発行日 2021-06-16
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| 出版者 |
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出版者 |
Copernicus Publications |
| 権利情報 |
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|
権利情報 |
This work is distributed under the Creative Commons Attribution 4.0 License. |
| EID |
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|
識別子 |
378545 |
| 言語 |
|
|
言語 |
eng |
aab_64_1_265.pdf (336 KB)
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