| Item type |
文献 / Documents(1) |
| 公開日 |
2021-12-07 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1177/0963689720954798 |
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関連名称 |
10.1177/0963689720954798 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
The Fragility of Cryopreserved Insulin-producing Cells Differentiated from Adipose-tissue-derived Stem Cells |
| 著者 |
徳田, 和憲
池本, 哲也
齋藤, 裕
宮崎, 克己
山下, 祥子
山田, 眞一郎
居村, 暁
森根, 裕二
島田, 光生
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| 抄録 |
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内容記述 |
The aim of our study is to determine whether insulin-producing cells (IPCs) differentiated from adipose-tissue-derived stem cells (ADSCs) can be cryopreserved. Human ADSCs were differentiated into IPCs using our two-step protocol encompassing a three-dimensional culture and xenoantigen-free method. Thereafter, IPCs were frozen using three different methods. First, IPCs were immediately frozen at −80°C (−80°C group). Second, IPCs were initially placed into a Bicell freezing container before freezing at −80°C (BICELL group). Third, a vitrification method for oocytes and embryos was used (CRYOTOP group). Cell counting kit-8 (CCK-8) assay showed that cell viability was decreased in all groups after cryopreservation (P < 0.01). Corroboratively, the amount of adenosine triphosphate was markedly decreased after cryopreservation in all groups (P < 0.01). Immunofluorescence staining showed a reduced positive staining area for insulin in all cryopreservation groups. Furthermore, 4′,6-diamidino-2-phenylindole and merged immunofluorescence images showed that cryopreserved cells appeared to be randomly reduced in the −80°C group and CRYOTOP group, while only the central region was visibly reduced in the BICELL group. Using immunohistochemical staining, IPCs after cryopreservation were shown to be positive for cleaved caspase-3 antibody in all groups. Finally, insulin secretion following glucose stimulation was significantly reduced in IPCs from all groups after cryopreservation (P < 0.01). In conclusion, IPCs may be too fragile for cryopreservation with accomplished methods and further investigations for a suitable preservation method are required. |
| キーワード |
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|
主題 |
insulin-producing cell |
| キーワード |
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|
主題 |
cryopreservation |
| キーワード |
|
|
主題 |
adipose-derived stem cell |
| キーワード |
|
|
主題 |
regenerative medicine |
| 書誌情報 |
en : Cell Transplantation
巻 29,
発行日 2020-09-02
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
09636897 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15553892 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1084767X |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11523185 |
| 出版者 |
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|
出版者 |
SAGE Publications |
| 権利情報 |
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|
権利情報 |
This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
| EID |
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|
識別子 |
376970 |
| 言語 |
|
|
言語 |
eng |
ct_29.pdf (1.45 MB)
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