WEKO3
-
RootNode
アイテム
FRET-Based Detection and Quantification of HIV-1 Virion Maturation
https://tokushima-u.repo.nii.ac.jp/records/2009426
https://tokushima-u.repo.nii.ac.jp/records/20094262f65e913-3aae-4518-841b-8cdbb4f5d295
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fmicb_12_647452.pdf (1.93 MB)
|
.png)
|
| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2021-12-22 | |||||||||||||||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.3389/fmicb.2021.647452 | |||||||||||||||||||||||||||||||||||||||
| 関連名称 | 10.3389/fmicb.2021.647452 | |||||||||||||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||||||||||||
| タイトル | FRET-Based Detection and Quantification of HIV-1 Virion Maturation | |||||||||||||||||||||||||||||||||||||||
| タイトル別表記 | ||||||||||||||||||||||||||||||||||||||||
| その他のタイトル | Quantitative Virion Maturation Fluorescence Microscopy | |||||||||||||||||||||||||||||||||||||||
| 著者 |
Sarca, Anamaria D.
× Sarca, Anamaria D.
× Sardo, Luca
× フクダ, ヒロフミ
× マツイ, ヒロユキ
× シラカワ, コウタロウ
× 堀川, 一樹
WEKO
586
× タカオリ コンドウ, アキフミ
× 泉, 泰輔 |
|||||||||||||||||||||||||||||||||||||||
| 抄録 | ||||||||||||||||||||||||||||||||||||||||
| 内容記述 | HIV-1 infectivity is achieved through virion maturation. Virus particles undergo structural changes via cleavage of the Gag polyprotein mediated by the viral protease, causing the transition from an uninfectious to an infectious status. The majority of proviruses in people living with HIV-1 treated with combination antiretroviral therapy are defective with large internal deletions. Defective proviral DNA frequently preserves intact sequences capable of expressing viral structural proteins to form virus-like particles whose maturation status is an important factor for chronic antigen-mediated immune stimulation and inflammation. Thus, novel methods to study the maturation capability of defective virus particles are needed to characterize their immunogenicity. To build a quantitative tool to study virion maturation in vitro, we developed a novel single virion visualization technique based on fluorescence resonance energy transfer (FRET). We inserted an optimized intramolecular CFP-YPF FRET donor-acceptor pair bridged with an HIV-1 protease cleavage sequence between the Gag MA-CA domains. This system allowed us to microscopically distinguish mature and immature virions via their FRET signal when the FRET donor and acceptor proteins were separated by the viral protease during maturation. We found that approximately 80% of the FRET labeled virus particles were mature with equivalent infectivity to wild type. The proportion of immature virions was increased by treatment of virus producer cells with a protease inhibitor in a dose-dependent manner, which corresponded to a relative decrease in infectivity. Potential areas of application for this tool are assessing maturation efficiency in different cell type settings of intact or deficient proviral DNA integrated cells. We believe that this FRET-based single-virion imaging platform will facilitate estimating the impact on the immune system of both extracellular intact and defective viruses by quantifying the Gag maturation status. | |||||||||||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||||||||||
| 主題 | HIV-1 Gag maturation | |||||||||||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||||||||||
| 主題 | Förster Resonance Energy Transfer | |||||||||||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||||||||||
| 主題 | single virion imaging | |||||||||||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||||||||||
| 主題 | protease inhibitor | |||||||||||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||||||||||
| 主題 | fluorescence microscopy | |||||||||||||||||||||||||||||||||||||||
| 書誌情報 |
en : Frontiers in Microbiology 巻 12, p. 647452, 発行日 2021-03-09 |
|||||||||||||||||||||||||||||||||||||||
| 収録物ID | ||||||||||||||||||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||||||||||||
| 収録物識別子 | 1664302X | |||||||||||||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||||||||||||
| 出版者 | Frontiers Media SA | |||||||||||||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||||||||||||
| 権利情報 | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||||||||||||||||||||||||||||||||||||
| EID | ||||||||||||||||||||||||||||||||||||||||
| 識別子 | 379800 | |||||||||||||||||||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||||||||||||||||||
