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STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells
https://tokushima-u.repo.nii.ac.jp/records/2009461
https://tokushima-u.repo.nii.ac.jp/records/2009461c3672ff0-5e9d-4b92-a9f3-96f80a91323f
| 名前 / ファイル | ライセンス | アクション |
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jraas_21_3.pdf (1.17 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||||
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| 公開日 | 2022-01-17 | |||||||||||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1177/1470320320946527 | |||||||||||||||||||||||||||||||
| 関連名称 | 10.1177/1470320320946527 | |||||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||||
| タイトル | STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells | |||||||||||||||||||||||||||||||
| 著者 |
Penrose, Harrison M
× Penrose, Harrison M
× カツラダ, アケミ
× ミヤタ, カヨコ
× 漆原, 真樹
WEKO
190
× サトウ, リョウスケ
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| 内容記述 | Objective: Intrarenal interferon-γ significantly contributes to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels are elevated. However, the exact nature of the role that interferon-γ plays in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully delineated. Therefore, the aim of this study was to investigate the role that interferon-γ plays in angiotensinogen and monocyte chemoattractant protein 1 expression. Methods: Primary cultured rat mesangial cells were treated with 0–20 ng/mL interferon-γ for 2, 8 or 24 hours. Expression levels of angiotensinogen, monocyte chemoattractant protein 1, suppressors of cytokine signaling 1, an intracellular suppressor of Janus kinase-signal transducers and activators of transcription signaling and activity of the Janus kinase-signal transducers and activators of transcription pathway were evaluated by reverse transcriptase polymerase chain reaction and western blot analysis. Results: Interferon-γ increased angiotensinogen expression in mesangial cells with maximal augmentation observed following 5 ng/mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). Further increases were reduced or absent using higher concentrations of interferon-γ. Following treatments, monocyte chemoattractant protein 1 expression was induced in a linear dose-dependent manner (6.85 ± 0.62-fold by 20 ng/mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressors of cytokine signaling 1 expression in a linear dose-dependent manner. The suppression of STAT1 and suppressors of cytokine signaling 1 expression by small interference RNAs facilitated an increase in interferon-γ-induced angiotensinogen expression, indicating that these two factors negatively regulate angiotensinogen expression. In contrast, the increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells. Conclusion: These results demonstrate that while interferon-γ increases both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 plays an opposing role in the regulation of each factor in mesangial cells. |
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| キーワード | ||||||||||||||||||||||||||||||||
| 主題 | Interferon-γ | |||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||
| 主題 | JAK-STAT pathway | |||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||
| 主題 | angiotensinogen | |||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||
| 主題 | MCP-1 | |||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||
| 主題 | mesangial cell | |||||||||||||||||||||||||||||||
| 書誌情報 |
en : Journal of the Renin-Angiotensin-Aldosterone System 巻 21, 号 3, 発行日 2020-08-03 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||||
| 収録物識別子 | 14703203 | |||||||||||||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||||
| 収録物識別子 | 17528976 | |||||||||||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||||||||
| 収録物識別子 | AA11699454 | |||||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||||
| 出版者 | SAGE Publications | |||||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||||
| 権利情報 | This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). | |||||||||||||||||||||||||||||||
| EID | ||||||||||||||||||||||||||||||||
| 識別子 | 380007 | |||||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||||
