| Item type |
文献 / Documents(1) |
| 公開日 |
2021-12-09 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.bbalip.2021.159088 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.bbalip.2021.159088 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Characterization of uptake and metabolism of very long-chain fatty acids in peroxisome-deficient CHO cells |
|
言語 |
en |
| 著者 |
Ali, Hanif
モリト, カツヤ
Hasi, Rumana Yesmin
(中橋)粟飯原, 睦美
林, 順司
川上, 竜巳
金丸, 芳
土屋, 浩一郎
サンゴ, カズノリ
田中, 保
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Fatty acids (FAs) longer than C20 are classified as very long-chain fatty acids (VLCFAs). Although biosynthesis and degradation of VLCFAs are important for the development and integrity of the myelin sheath, knowledge on the incorporation of extracellular VLCFAs into the cells is limited due to the experimental difficulty of solubilizing them. In this study, we found that a small amount of isopropanol solubilized VLCFAs in aqueous medium by facilitating the formation of the VLCFA/albumin complex. Using this solubilizing technique, we examined the role of the peroxisome in the uptake and metabolism of VLCFAs in Chinese hamster ovary (CHO) cells. When wild-type CHO cells were incubated with saturated VLCFAs (S-VLCFAs), such as C23:0 FA, C24:0 FA, and C26:0 FA, extensive uptake was observed. Most of the incorporated S-VLCFAs were oxidatively degraded without acylation into cellular lipids. In contrast, in peroxisome-deficient CHO cells uptake of S-VLCFAs was marginal and oxidative metabolism was not observed. Extensive uptake and acylation of monounsaturated (MU)-VLCFAs, such as C24:1 FA and C22:1 FA, were observed in both types of CHO cells. However, oxidative metabolism was evident only in wild-type cells. Similar manners of uptake and metabolism of S-VLCFAs and MU-VLCFAs were observed in IFRS1, a Schwan cell-derived cell line. These results indicate that peroxisome-deficient cells limit intracellular S-VLCFAs at a low level by halting uptake, and as a result, peroxisome-deficient cells almost completely lose the clearance ability of S-VLCFAs accumulated outside of the cells. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Peroxisome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Very long-chain fatty acids |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Beta-oxidation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Peroxisome disease |
| 書誌情報 |
en : Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
巻 1867,
号 2,
p. 159088,
発行日 2021-11-27
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13881981 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11522965 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11300409 |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
383342 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
bbalip_1867_2_159088.pdf (1.2 MB)
