WEKO3
アイテム
Genome editing in mammalian cells using the CRISPR type I-D nuclease
https://tokushima-u.repo.nii.ac.jp/records/2009513
https://tokushima-u.repo.nii.ac.jp/records/200951350c622db-7886-43b0-934e-55945e34c91e
| 名前 / ファイル | ライセンス | アクション |
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nar_49_11_6347.pdf (6.05 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2022-01-28 | |||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1093/nar/gkab348 | |||||||||||||||||||||||
| 関連名称 | 10.1093/nar/gkab348 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Genome editing in mammalian cells using the CRISPR type I-D nuclease | |||||||||||||||||||||||
| 著者 |
刑部, 敬史
× 刑部, 敬史
WEKO
664
× 和田, 直樹× ムラカミ, エミ
× ミヤシタ, ナオユキ
× 刑部, 祐里子 |
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| 抄録 | ||||||||||||||||||||||||
| 内容記述 | Adoption of CRISPR–Cas systems, such as CRISPR–Cas9 and CRISPR–Cas12a, has revolutionized genome engineering in recent years; however, application of genome editing with CRISPR type I—the most abundant CRISPR system in bacteria—remains less developed. Type I systems, such as type I-E, and I-F, comprise the CRISPR-associated complex for antiviral defense (‘Cascade’: Cas5, Cas6, Cas7, Cas8 and the small subunit) and Cas3, which degrades the target DNA; in contrast, for the sub-type CRISPR–Cas type I-D, which lacks a typical Cas3 nuclease in its CRISPR locus, the mechanism of target DNA degradation remains unknown. Here, we found that Cas10d is a functional nuclease in the type I-D system, performing the role played by Cas3 in other CRISPR–Cas type I systems. The type I-D system can be used for targeted mutagenesis of genomic DNA in human cells, directing both bi-directional long-range deletions and short insertions/deletions. Our findings suggest the CRISPR–Cas type I-D system as a unique effector pathway in CRISPR that can be repurposed for genome engineering in eukaryotic cells. | |||||||||||||||||||||||
| 書誌情報 |
en : Nucleic Acids Research 巻 49, 号 11, p. 6347-6363, 発行日 2021-06-02 |
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| 収録物ID | ||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 13624962 | |||||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 03051048 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA00760269 | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | Oxford University Press | |||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||
| 権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com | |||||||||||||||||||||||
| EID | ||||||||||||||||||||||||
| 識別子 | 376262 | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
