| Item type |
文献 / Documents(1) |
| 公開日 |
2022-02-03 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1126/sciadv.abd2102 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1126/sciadv.abd2102 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Full-field fluorescence lifetime dual-comb microscopy using spectral mapping and frequency multiplexing of dual-comb optical beats |
|
言語 |
en |
| 著者 |
水野, 孝彦
長谷, 栄治
南川, 丈夫
時実, 悠
オエ
コレサワ
山本, 裕紹
安井, 武史
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust to photobleaching. However, since most FLIMs are based on point-to-point measurements, mechanical scanning of a focal spot is needed for forming an image, which hampers rapid imaging. Here, we demonstrate scan-less full-field FLIM based on a one-to-one correspondence between two-dimensional (2D) image pixels and frequency-multiplexed radio frequency (RF) signals. A vast number of dual-comb optical beats between dual optical frequency combs are effectively adopted for 2D spectral mapping and high-density frequency multiplexing in the RF region. Bimodal images of fluorescence amplitude and lifetime are obtained with high quantitativeness from amplitude and phase spectra of fluorescence RF comb modes without the need for mechanical scanning. The parallelized FLIM will be useful for rapid quantitative fluorescence imaging in life science. |
|
言語 |
en |
| 書誌情報 |
en : Science Advances
巻 7,
号 1,
p. eabd2102,
発行日 2021-01-01
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
23752548 |
| 出版者 |
|
|
出版者 |
American Association for the Advancement of Science |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). |
| EID |
|
|
識別子 |
377692 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
sciadv_7_1_eabd2102.pdf (4.63 MB)
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