| Item type |
文献 / Documents(1) |
| 公開日 |
2022-03-08 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1016/j.bbrep.2021.101067 |
|
|
関連名称 |
10.1016/j.bbrep.2021.101067 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Rapid modification of antibodies on the surface of liposomes composed of high-affinity protein A-conjugated phospholipid for selective drug delivery |
| 著者 |
ハマ, ススム
サカイ, ミカ
イタクラ, ショウコ
マジマ, エイジ
小暮, 健太朗
|
| 抄録 |
|
|
内容記述 |
Antibody-modified liposomes, immuno-liposomes, can selectively deliver encapsulated drug ‘cargos’ to cells via the interaction of cell surface proteins with antibodies. However, chemical modification of both the antibodies and phospholipids is required for the preparation of immuno-liposomes for each target protein using conventional methods, which is time-consuming. In the present study, we demonstrated that high-affinity protein A- (Protein A-R28: PAR28) displaying liposomes prepared by the post-insertion of PAR28-conjugated phospholipid through polyethylene glycol (PEG)-linkers (PAR28-PEG-lipo) can undergo rapid modification of antibodies on their surface, and the liposomes can be delivered to cells based on their modified antibodies. Anti-CD147 and anti-CD31 antibodies could be modified with PAR28-PEG-lipo within 1 h, and each liposome was specifically taken up by CD147- and CD31-positive cells, respectively. The cellular amounts of doxorubicin delivered by anti-CD147 antibody-modified PAR28-PEG-lipo were significantly higher than those of isotype control antibody-modified liposomes. PAR28-PEG-lipo can easily and rapidly undergo modification of various antibodies on their surface, which then makes them capable of selective drug delivery dependent on the antibodies. |
| キーワード |
|
|
主題 |
Immuno-liposomes |
| キーワード |
|
|
主題 |
Drug delivery |
| キーワード |
|
|
主題 |
Antibody |
| キーワード |
|
|
主題 |
Protein A |
| キーワード |
|
|
主題 |
Cancer |
| 書誌情報 |
en : Biochemistry and Biophysics Reports
巻 27,
p. 101067,
発行日 2021-07-02
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
24055808 |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
377806 |
| 言語 |
|
|
言語 |
eng |
bbrep_27_101067.pdf (2.54 MB)
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