| Item type |
文献 / Documents(1) |
| 公開日 |
2022-03-08 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/cells10112827 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3390/cells10112827 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections |
|
言語 |
en |
| 著者 |
タドコロ, ススム
トクヤマ トダ, レイコ
舘原, 誠晃
イデ, シンジ
ウメキ, ヒロチカ
三好, 圭子
野間, 隆文
里村, 一人
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Considering that every tissue/organ has the most suitable microenvironment for its functional cells, controlling induced pluripotent stem cell (iPSC) differentiation by culture on frozen sections having a suitable microenvironment is possible. Induced PSCs were cultured on frozen sections of the liver, the brain, the spinal cord, and cover glasses (control) for 9 days. The iPSCs cultured on the sections of the liver resembled hepatocytes, whereas those on sections of the brain and the spinal cord resembled neuronal cells. The percentage of hepatocytic marker-positive cells in the iPSCs cultured on the sections of the liver was statistically higher than that of those in the iPSCs cultured on the sections of the brain and the spinal cord or on cover glasses. In contrast, the iPSCs cultured on the sections of the brain and the spinal cord revealed a high percentage of neural marker-positive cells. Thus, iPSCs can be differentiated into a specific cell lineage in response to specific factors within frozen sections of tissues/organs. Differentiation efficacy of the frozen sections markedly differed between the iPSC clones. Therefore, our induction method could be simple and effective for evaluating the iPSC quality. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
iPSC |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
differentiation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
frozen section |
| 書誌情報 |
en : Cells
巻 10,
号 11,
p. 2827,
発行日 2021-10-21
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20734409 |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
382799 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
cells_10_11_2827.pdf (2.58 MB)
.png)
