| Item type |
文献 / Documents(1) |
| 公開日 |
2022-04-13 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1074/JBC.RA120.015861 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1074/JBC.RA120.015861 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Iron uptake mediated by the plant-derived chelator nicotianamine in the small intestine |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Effects of nicotianamine on iron in the small intestine |
|
言語 |
en |
| 著者 |
ムラタ, ヨシコ
ヨシダ, マサミ
サカモト, ナホ
モリモト, シホ
ワタナベ, タケヒロ
難波, 康祐
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Iron is an essential metal for all living organisms that is absorbed in the intestinal cells as a heme-chelated or free form. It is unclear how important plant-derived chelators, such as nicotianamine (NA), an organic small molecule that is ubiquitous in crops, vegetables, and various other foods, contribute to iron bioavailability in mammals. We performed electrophysiological assays with Xenopus laevis oocytes and radioactive tracer experiments with Caco-2 cells. The findings revealed that the proton-coupled amino acid transporter SLC36A1 (PAT1) transports iron in the form of NA-Fe (II) complex in vitro. Decreased expression of hPAT1 by RNA interference in Caco-2 cells reduced the uptake of NA-59Fe (II) complex. The uptake of inorganic 59Fe (II) was relatively unaffected. These results imply that PAT1 transports iron as a NA-Fe (II) complex. The rate of 59Fe absorption in the spleen, liver, and kidney was higher when mice were orally administered NA-59Fe (II) compared with free 59Fe (II). The profile of site-specific PAT1 expression in the mouse intestine coincided with those of NA and iron contents, which were the highest in the proximal jejunum. Orally administered NA-59Fe (II) complex in mice was detected in the proximal jejunum by thin layer chromatography. In contrast, much less 59Fe (or NA) was detected in the duodenum, where the divalent metal transporter SLC11A2 (DMT1) absorbs free Fe (II). The collective results revealed the role of PAT1 in NA-Fe (II) absorption in the intestine and potential implication of NA in iron uptake in mammals. |
|
言語 |
en |
| 書誌情報 |
en : Journal of Biological Chemistry
巻 296,
p. 100195,
発行日 2021-01-04
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00219258 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1202441X |
| 出版者 |
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|
出版者 |
American Society for Biochemistry and Molecular Biology |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
381727 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jbc_296_100195.pdf (1.62 MB)
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