| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2022-04-13 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.3390/jcm10030386 |
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関連名称 |
10.3390/jcm10030386 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Gan-Lu-Yin (Kanroin), Traditional Chinese Herbal Extracts, Reduces Osteoclast Differentiation In Vitro and Prevents Alveolar Bone Resorption in Rat Experimental Periodontitis |
| 著者 |
稲垣, 裕司
木戸, 淳一
西川, 泰史
木戸, 理恵
坂本, 英次郎
板東, 美香
成石, 浩司
永田, 俊彦
湯本, 浩通
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| 抄録 |
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内容記述 |
Gan-Lu-Yin (GLY), a traditional Chinese herbal medicine, shows therapeutic effects on periodontitis, but that mechanism is not well known. This study aims to clarify the precise mechanism by investigating the inhibitory effects of GLY extracts on osteoclastogenesis in vitro and on bone resorption in periodontitis in vivo. RAW264.7 cells are cultured with soluble receptor activator of nuclear factor-kappa B (sRANKL) and GLY extracts (0.01–1.0 mg/mL), and stained for tartrate-resistant acid phosphatase (TRAP) to evaluate osteoclast differentiation. Experimental periodontitis is induced by placing a nylon ligature around the second maxillary molar in rats, and rats are administered GLY extracts (60 mg/kg) daily for 20 days. Their maxillae are collected on day 4 and 20, and the levels of alveolar bone resorption and osteoclast differentiation are estimated using micro-computed tomography (CT) and histological analysis, respectively. In RAW264.7 cells, GLY extracts significantly inhibit sRANKL-induced osteoclast differentiation at a concentration of more than 0.05 mg/mL. In experimental periodontitis, administering GLY extracts significantly decreases the number of TRAP-positive osteoclasts in the alveolar bone on day 4, and significantly inhibits the ligature-induced bone resorption on day 20. These results show that GLY extracts suppress bone resorption by inhibiting osteoclast differentiation in experimental periodontitis, suggesting that GLY extracts are potentially useful for oral care in periodontitis. |
| キーワード |
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主題 |
Gan-Lu-Yin |
| キーワード |
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主題 |
periodontitis |
| キーワード |
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主題 |
herbal medicine |
| キーワード |
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主題 |
osteoclastogenesis |
| 書誌情報 |
en : Journal of Clinical Medicine
巻 10,
号 3,
p. 386,
発行日 2021-01-20
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20770383 |
| 出版者 |
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出版者 |
MDPI |
| 権利情報 |
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権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
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識別子 |
379180 |
| 言語 |
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言語 |
eng |
jcm_10_3_386.pdf (3.22 MB)
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