| Item type |
文献 / Documents(1) |
| 公開日 |
2022-04-20 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3389/fmolb.2022.811548 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3389/fmolb.2022.811548 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Genetic and Functional Analyses of Archaeal ATP-Dependent RNA Ligase in C/D Box sRNA Circularization and Ribosomal RNA Processing |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Archaea ATP-Dependent RNA Ligase |
|
言語 |
en |
| 著者 |
Liu, Yancheng
タカギ, ユウコ
Sugijanto, Milyadi
Duong My Nguyen, Kieu
平田, 章
ホリ, ヒロユキ
Ho, C. Kiong
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
RNA ligases play important roles in repairing and circularizing RNAs post-transcriptionally. In this study, we generated an allelic knockout of ATP-dependent RNA ligase (Rnl) in the hyperthermophilic archaeon Thermococcus kodakarensis to identify its biological targets. A comparative analysis of circular RNA reveals that the Rnl-knockout strain represses circularization of C/D box sRNAs without affecting the circularization of tRNA and rRNA processing intermediates. Recombinant archaeal Rnl could circularize C/D box sRNAs with a mutation in the conserved C/D box sequence element but not when the terminal stem structures were disrupted, suggesting that proximity of the two ends could be critical for intramolecular ligation. Furthermore, T. kodakarensis accumulates aberrant RNA fragments derived from ribosomal RNA in the absence of Rnl. These results suggest that Rnl is responsible for C/D box sRNA circularization and may also play a role in ribosomal RNA processing. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
circular RNA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
RNA ligase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
thermococcus kodakarensis KOD1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
rRNA processing |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
C/D box sRNAs |
| 書誌情報 |
en : Frontiers in Molecular Biosciences
巻 9,
p. 811548,
発行日 2022-03-25
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2296889X |
| 出版者 |
|
|
出版者 |
Frontiers Media S.A. |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| EID |
|
|
識別子 |
385151 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
fmolb_9_811548.pdf (2.09 MB)
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