| Item type |
文献 / Documents(1) |
| 公開日 |
2022-05-11 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1038/s41594-021-00665-8 |
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|
言語 |
ja |
|
|
関連名称 |
10.1038/s41594-021-00665-8 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The tertiary structure of the human Xkr8–Basigin complex that scrambles phospholipids at plasma membranes |
|
言語 |
en |
| 著者 |
サクラギ, タカハル
カナイ, リュウタ
ツツミ, アキヒサ
ナリタ, ヒロタカ
オオニシ, エリコ
ニシノ, コウヘイ
ミヤザキ, タクヤ
ババ, タケシ
小迫, 英尊
ナカガワ, アツシ
キッカワ, マサヒデ
トヨシマ, チカシ
ナガタ, シゲカズ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Xkr8–Basigin is a plasma membrane phospholipid scramblase activated by kinases or caspases. We combined cryo-EM and X-ray crystallography to investigate its structure at an overall resolution of 3.8 Å. Its membrane-spanning region carrying 22 charged amino acids adopts a cuboid-like structure stabilized by salt bridges between hydrophilic residues in transmembrane helices. Phosphatidylcholine binding was observed in a hydrophobic cleft on the surface exposed to the outer leaflet of the plasma membrane. Six charged residues placed from top to bottom inside the molecule were essential for scrambling phospholipids in inward and outward directions, apparently providing a pathway for their translocation. A tryptophan residue was present between the head group of phosphatidylcholine and the extracellular end of the path. Its mutation to alanine made the Xkr8–Basigin complex constitutively active, indicating that it plays a vital role in regulating its scramblase activity. The structure of Xkr8–Basigin provides insights into the molecular mechanisms underlying phospholipid scrambling. |
|
言語 |
en |
| 書誌情報 |
en : Nature Structural & Molecular Biology
巻 28,
号 10,
p. 825-834,
発行日 2021-10-08
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15459993 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15459985 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11879900 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11952261 |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
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|
識別子 |
383891 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
nsmb_28_10_825.pdf (9.36 MB)
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