| Item type |
文献 / Documents(1) |
| 公開日 |
2022-05-31 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
関連識別子 |
https://doi.org/10.1002/vms3.659 |
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関連名称 |
10.1002/vms3.659 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Zona pellucida treatment before CRISPR/Cas9-mediated genome editing of porcine zygotes |
| 著者 |
Namula, Zhao
平田, 真樹
Le, Quynh Anh
Lin, Qingyi
タケバヤシ, コウキ
ヨシムラ, ナオアキ
谷原, 史倫
ソンキテイデイロ, チヨンマナート
| ja |
ソンキテイデイロ, チヨンマナート
|
| ja-Kana |
トンキッチィディロク, チヨンマナート
|
| en |
Thongkittidilok, Chommanart
|
Search repository
音井, 威重
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| 抄録 |
|
|
内容記述 |
Background: Increasing the permeability of the zona pellucida (ZP) of oocytes before CRISPR/Cas9 electroporation may improve the efficiency of gene editing; however, the effects of this approach on subsequent developmental processes are unclear. In this study, the effects of ZP treatment before electroporation on embryonic development and gene editing in porcine embryos were evaluated.
Methods: The ZP of zygotes was weakened or removed by exposure to 0.5% actinase E, followed by electroporation of the Cas9 protein with guide RNA targeting GGTA1.
Results: The blastocyst formation rate of ZP-free zygotes after electroporation was significantly lower (p < 0.05) than that of ZP-intact zygotes. The mutation rate in blastocysts from ZP-weakened zygotes was similar to that in ZP-intact zygotes, whereas ZP removal increased the mutation rate. The mutation efficiency in blastocysts from electroporated zygotes did not differ among ZP treatment groups.
Conclusions: Our results indicate that weakening the ZP does not affect the developmental competence, mutation rate, or mutation efficiency of electroporated zygotes, whereas ZP removal has a detrimental effect on embryonic development but may increase the mutation rate. |
| キーワード |
|
|
主題 |
CRISPR |
| キーワード |
|
|
主題 |
electroporation |
| キーワード |
|
|
主題 |
genome editing |
| キーワード |
|
|
主題 |
porcine zygote |
| キーワード |
|
|
主題 |
zona pellucida |
| 書誌情報 |
en : Veterinary Medicine and Science
巻 8,
号 1,
p. 164-169,
発行日 2021-10-21
|
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20531095 |
| 出版者 |
|
|
出版者 |
John Wiley & Sons |
| 権利情報 |
|
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
| EID |
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|
識別子 |
384278 |
| 言語 |
|
|
言語 |
eng |
vms_8_1_164.pdf (778 KB)
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