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Precipitant-Free Crystallization of Lysozyme and Glucose Isomerase by Drying
https://tokushima-u.repo.nii.ac.jp/records/2009869
https://tokushima-u.repo.nii.ac.jp/records/2009869c5c52183-0927-4503-a6f5-bde0562634d5
| 名前 / ファイル | ライセンス | アクション |
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cryst_12_2_129.pdf (994 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2022-06-02 | |||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.3390/cryst12020129 | |||||||||||||||||||||||
| 関連名称 | 10.3390/cryst12020129 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Precipitant-Free Crystallization of Lysozyme and Glucose Isomerase by Drying | |||||||||||||||||||||||
| 著者 |
鈴木, 良尚
× 鈴木, 良尚
WEKO
63
× フジワラ, シオリ
× 上田, 昭子
WEKO
375
× 坂井, 隆志 |
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| 内容記述 | Protein crystallization is usually conducted by using precipitants, although the “salting-out” phenomenon is still unclear and complex. Moreover, the addition of precipitants sometimes results in irreversible disordered precipitation of protein molecules. Although precipitant-free lysozyme crystals obtained by centrifugal concentration showed significant changes in three-dimensional structure compared to the structure of salted-out crystals, it was rather difficult to mount crystals from a viscous dense liquid phase after centrifugal concentration, and the quality of the crystals often deteriorated during the mounting process. Here we present novel precipitant-free crystallization methods, which were effective for lysozyme and glucose isomerase. Tetragonal lysozyme crystals were successfully crystallized in a glass capillary simply by drying highly concentrated lysozyme solution in the presence of 0.01 M hydrochloric acid without using any precipitants. Glucose isomerase dissolved in ultra-pure water was also successfully crystallized in hanging drops by drying highly concentrated solution under low-humidity conditions. Oscillation images of the obtained crystals were safely collected without handling; they clearly indicated the crystals had a tetragonal form for lysozyme and an orthorhombic form for glucose isomerase, and their lattice parameters are similar to those of previously reported crystals obtained by salting-out methods. | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | precipitant-free | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | drying | |||||||||||||||||||||||
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| 主題 | highly concentrated solution | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | oscillation image | |||||||||||||||||||||||
| 書誌情報 |
en : Crystals 巻 12, 号 2, p. 129, 発行日 2022-01-18 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 20734352 | |||||||||||||||||||||||
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| 出版者 | MDPI | |||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||
| 権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||||||||||||||||||||
| EID | ||||||||||||||||||||||||
| 識別子 | 383858 | |||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||
