| Item type |
文献 / Documents(1) |
| 公開日 |
2022-06-01 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
循環血中遊離DNAを用いた膵β細胞傷害の新規検出法の確立 |
| タイトル別表記 |
|
|
その他のタイトル |
Novel method for detection of pancreatic beta cell death using cell-free DNA |
| 著者 |
岡田, 朝美
山田, 美鈴
森, 博康
明比, 祐子
倉橋, 清衛
吉田, 守美子
遠藤, 逸朗
粟飯原, 賢一
松久, 宗英
黒田, 暁生
|
| 抄録 |
|
|
内容記述 |
In people with type１ diabetes（T１D）, biomarkers that can sensitively and quantitatively evaluate injury of pancreatic beta cell are required in order to predict the onset of the disease at an early stage and to provide interventions to prevent the progression of the disease. We developed a new method for quantifying pancreatic beta cell-derived insulin DNA in circulation that combines bisulfite conversion and Amplification Refractory Mutation System（ARMS）PCR, which can be performed using a conventional real-time PCR system. We applied this method to T１D patients and healthy adults, both could be detected in about ３０％ of cases. The results in healthy adults indicate that this method may have sensitivity to detect the turnover of pancreatic beta cells at physiological conditions. In post-onset T１D patients, there were many negatives because the amount of residual pancreatic beta cells was extremely small. However, in some cases with a short duration of the disease, pancreatic beta cell-derived insulin DNA was detected in negative correlation between the duration of the disease, that suggested the residual pancreatic beta cells continue to be slowly destroyed. It was demonstrated that the time course of pathophysiology in T１D could be understood using this method. |
| キーワード |
|
|
主題 |
Insulin |
| キーワード |
|
|
主題 |
DNA methylation |
| キーワード |
|
|
主題 |
RT-PCR |
| キーワード |
|
|
主題 |
Type１ diabetes |
| キーワード |
|
|
主題 |
cell-free DNA |
| 書誌情報 |
ja : 四国医学雑誌
en : Shikoku Acta Medica
巻 77,
号 5-6,
p. 249-254,
発行日 2021-12-25
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00373699 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00102041 |
| 出版者 |
|
|
出版者 |
徳島医学会 |
| EID |
|
|
識別子 |
386062 |
| 言語 |
|
|
言語 |
jpn |
sam_77_5-6_249.pdf (1.38 MB)
