| Item type |
文献 / Documents(1) |
| 公開日 |
2022-07-22 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/ijms23063357 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3390/ijms23063357 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Assessment of Ultra-Early-Stage Liver Fibrosis in Human Non-Alcoholic Fatty Liver Disease by Second-Harmonic Generation Microscopy |
|
言語 |
en |
| 著者 |
南川, 丈夫
長谷, 栄治
(市村)清水, 真祐子
モリモト, ユウキ
スズキ, アキヒロ
安井, 武史
ナカムラ, サトコ
ツツイ, アケミ
タカグチ, コウイチ
常山, 幸一
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Non-alcoholic fatty liver disease (NAFLD) is associated with the chronic progression of fibrosis. In general, the progression of liver fibrosis is determined by a histopathological assessment with a collagen-stained section; however, the ultra-early stage of liver fibrosis is challenging to identify because of the low sensitivity in the collagen-selective staining method. In the present study, we demonstrate the feasibility of second-harmonic generation (SHG) microscopy in the histopathological diagnosis of the liver of NAFLD patients for the quantitative assessment of the ultra-early stage of fibrosis. We investigated four representative NAFLD patients with early stages of fibrosis. SHG microscopy visualised well-matured fibrotic structures and early fibrosis diffusely involving liver tissues, whereas early fibrosis is challenging to be identified by conventional histopathological methods. Furthermore, the SHG emission directionality analysis revealed the maturation of each collagen fibre of each patient. As a result, SHG microscopy is feasible for assessing liver fibrosis on NAFLD patients, including the ultra-early stage of liver fibrosis that is difficult to diagnose by the conventional histopathological method. The assessment method of the ultra-early fibrosis by using SHG microscopy may serve as a crucial means for pathological, clinical, and prognostic diagnosis of NAFLD patients. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
second-harmonic generation microscopy |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
non-alcoholic fatty liver disease |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
non-alcoholic steatohepatitis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
fibrosis |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 23,
号 6,
p. 3357,
発行日 2022-03-20
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14220067 |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
384935 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ijms_23_6_3357.pdf (4.3 MB)
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