WEKO3
-
RootNode
アイテム
Development of a Nanocarrier-Based Splenic B Cell-Targeting System for Loading Antigens in Vitro
https://tokushima-u.repo.nii.ac.jp/records/2010065
https://tokushima-u.repo.nii.ac.jp/records/20100651bccb59a-4d6e-4132-8fab-dce1cd2372a1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bpb_45_7_926.pdf (1.01 MB)
|
|
| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2022-07-11 | |||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1248/bpb.b22-00222 | |||||||||||||||||||||||
| 関連名称 | 10.1248/bpb.b22-00222 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Development of a Nanocarrier-Based Splenic B Cell-Targeting System for Loading Antigens in Vitro | |||||||||||||||||||||||
| 著者 |
カワグチ, ヨシノ
× カワグチ, ヨシノ
× 清水, 太郎× 安藤, 英紀
WEKO
700
× 異島, 優× 石田, 竜弘
WEKO
1145
|
|||||||||||||||||||||||
| 抄録 | ||||||||||||||||||||||||
| 内容記述 | B cells are types of lymphocytes that are involved in the production of antibodies against pathogens. They also deliver and present antigens for the priming of T cells. Recently, we developed an in vivo splenic marginal zone (MZ) B cell-targeting liposomes decorated with polyethylene glycol (PEG) containing a hydroxyl-terminus group (HO-PEG-Lip). In an expansion of a previous study, we used HO-PEG-Lip as an in vitro antigen delivery tool to splenic B cells to test the ability of this formulation to overcome the limitations of the poor antigen uptake ability of B cells for implantation. To achieve our purpose, various factors were optimized. These factors include cell number, liposome concentration, pre-opsonization of liposomes, fresh serum concentration, and incubation time, all of which affect the extent of interaction between liposomes and B cells. As a result, we confirmed that the HO-PEG-Lip required incubation at 37 °C for at least 20 min with 50% mouse fresh serum followed by a subsequent incubation at 37 °C for at least another 30 min with splenic B cells. By using such a loading system, fluorescein isothiocyanate (FITC)-labeled ovalbumin (OVA), a model antigen, encapsulated in HO-PEG-Lip could be efficiently loaded into splenic B cells. In addition, HO-PEG-Lip and FITC-labeled OVA encapsulated in HO-PEG-Lip were efficiently associated with MZ-B cells with high levels of complement receptors (CRs) rather than follicular B cells with low levels of CRs. These results propose a novel and useful system to efficiently load antigens into B cells in vitro by taking advantage of complement systems. | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | B cell-based therapy | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | liposome | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | complement system | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | complement receptor | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | anti-polyethylene glycol (PEG) antibody | |||||||||||||||||||||||
| 書誌情報 |
en : Biological and Pharmaceutical Bulletin 巻 45, 号 7, p. 926-933, 発行日 2022-07-01 |
|||||||||||||||||||||||
| 収録物ID | ||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 13475215 | |||||||||||||||||||||||
| 収録物ID | ||||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA11696048 | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | The Pharmaceutical Society of Japan | |||||||||||||||||||||||
| EID | ||||||||||||||||||||||||
| 識別子 | 385361 | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
