| Item type |
文献 / Documents(1) |
| 公開日 |
2022-08-10 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.3892/ijo.2021.5239 |
|
|
関連名称 |
10.3892/ijo.2021.5239 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Cancer‑associated fibroblast‑induced M2‑polarized macrophages promote hepatocellular carcinoma progression via the plasminogen activator inhibitor‑1 pathway |
| タイトル別表記 |
|
|
その他のタイトル |
CAF-INDUCED TAMs PROMOTE HCC PROGRESSION VIA PAI-1 |
| 著者 |
Chen, Shuhai
森根, 裕二
徳田, 和憲
山田, 眞一郎
齋藤, 裕
西, 正暁
池本, 哲也
島田, 光生
|
| 抄録 |
|
|
内容記述 |
Targeting the tumor stroma is an important strategy in cancer treatment. Cancer‑associated fibroblasts (CAFs) and tumor‑associated macrophages (TAMs) are two main components in the tumor microenvironment (TME) in hepatocellular carcinoma (HCC), which can promote tumor progression. Plasminogen activator inhibitor‑1 (PAI‑1) upregulation in HCC is predictive of unfavorable tumor behavior and prognosis. However, the crosstalk between cancer cells, TAMs and CAFs, and the functions of PAI‑1 in HCC remain to be fully investigated. In the present study, macrophage polarization and key paracrine factors were assessed during their interactions with CAFs and cancer cells. Cell proliferation, wound healing and Transwell and Matrigel assays were used to investigate the malignant behavior of HCC cells in vitro. It was found that cancer cells and CAFs induced the M2 polarization of TAMs by upregulating the mRNA expression levels of CD163 and CD206, and downregulating IL‑6 mRNA expression and secretion in the macrophages. Both TAMs derived from cancer cells and CAFs promoted HCC cell proliferation and invasion. Furthermore, PAI‑1 expression was upregulated in TAMs after being stimulated with CAF‑conditioned medium and promoted the malignant behavior of the HCC cells by mediating epithelial‑mesenchymal transition. CAFs were the main producer of C‑X‑C motif chemokine ligand 12 (CXCL12) in the TME and CXCL12 contributed to the induction of PAI‑1 secretion in TAMs. In conclusion, the results of the present study suggested that CAFs promoted the M2 polarization of macrophages and induced PAI‑1 secretion via CXCL12. Furthermore, it was found that PAI‑1 produced by the TAMs enhanced the malignant behavior of the HCC cells. Therefore, these factors may be targets for inhibiting the crosstalk between tumor cells, CAFs and TAMs. |
| キーワード |
|
|
主題 |
tumor‑associated macrophages |
| キーワード |
|
|
主題 |
cancer‑associated fibroblasts |
| キーワード |
|
|
主題 |
hepatocellular carcinoma |
| キーワード |
|
|
主題 |
plasminogen activator inhibitor‑1 |
| キーワード |
|
|
主題 |
epithelial‑mesenchymal transition |
| 書誌情報 |
en : International Journal of Oncology
巻 59,
号 2,
p. 59,
発行日 2021-07-01
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
10196439 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
17912423 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10992511 |
| 出版者 |
|
|
出版者 |
Spandidos Publications |
| 権利情報 |
|
|
権利情報 |
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License. |
| EID |
|
|
識別子 |
386672 |
| 言語 |
|
|
言語 |
eng |
ijo_59_2_59.pdf (1.43 MB)
.png)
