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Development of vibration mechanical stimuli loading device for live cell fluorescence microscopy
https://tokushima-u.repo.nii.ac.jp/records/2010154
https://tokushima-u.repo.nii.ac.jp/records/2010154116f0302-09e5-4adf-b6ac-593b601a05bc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
jbse_17_2_21-00294.pdf (1.57 MB)
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| Item type | 文献 / Documents(1) | |||||||||||
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| 公開日 | 2022-08-18 | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 出版社版DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1299/jbse.21-00294 | |||||||||||
| 言語 | ja | |||||||||||
| 関連名称 | 10.1299/jbse.21-00294 | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Development of vibration mechanical stimuli loading device for live cell fluorescence microscopy | |||||||||||
| 言語 | en | |||||||||||
| 著者 |
佐藤, 克也
× 佐藤, 克也× オオモリ, ダイキ
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Bone metabolism is regulated by mechanical stimuli such as gravity and exercise. If bone formation can be promoted by artificial mechanical stimulation, the quality of life of the aging society can be improved, such as by reducing the progression of osteoporosis and preventing patients from being bedridden. Micro-vibration stimulation with frequencies of 15 Hz to 90 Hz and acceleration amplitudes of 0.1 g to 0.3 g is effectively promotes bone formation in animal models. However, the mechanism of how these weak vibrations are sensed by bone cells and are used to regulate bone metabolism is unknown. In this study, we developed a device to apply micro-vibration stimuli to osteoblasts cultured on a glass bottom dish and observed the cell response to the stimuli using a confocal laser scanning fluorescence microscope. The device performance was confirmed using driving tests, and the calcium signaling response of osteoblasts to micro-vibration stimuli was observed in real time and in situ. The calcium signaling response characteristics of the cells differed when the cells were subjected to 45 Hz and 90 Hz micro-vibration stimuli, even under a constant acceleration amplitude of 0.2 g. | |||||||||||
| 言語 | en | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Vibration stimuli | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Osteoblast | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Calcium signaling | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Cell biomechanics | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Mechanotransduction | |||||||||||
| 書誌情報 |
en : Journal of Biomechanical Science and Engineering 巻 17, 号 2, p. 21-00294, 発行日 2022 |
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| 収録物ID | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 18809863 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | The Japan Society of Mechanical Engineers | |||||||||||
| 言語 | en | |||||||||||
| 権利情報 | ||||||||||||
| 言語 | en | |||||||||||
| 権利情報 | This is an open access article under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/). | |||||||||||
| EID | ||||||||||||
| 識別子 | 385388 | |||||||||||
| 識別子タイプ | URI | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
