| Item type |
文献 / Documents(1) |
| 公開日 |
2022-08-19 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1111/php.13137 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1111/php.13137 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Enzymatic Conversion of Cypridina Luciferyl Sulfate to Cypridina Luciferin with Coenzyme A as a Sulfate Acceptor in Cypridina (Vargula) hilgendorfii |
|
言語 |
en |
| 著者 |
中村, 光裕
マツダ, カズオ
ナカムラ, ミサキ
ヤマシタ, キョウヘイ
スズキ, トモコ
イノウエ, サトシ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
In the luminous ostracod Cypridina (presently Vargula) hilgendorfii, Cypridina luciferyl sulfate (3-enol sulfate of Cypridina luciferin) is converted to Cypridina luciferin by a sulfotransferase with 3′-phosphoadenosine-5′-phosphate (PAP) as a sulfate acceptor. The resultant Cypridina luciferin is used for the luciferase–luciferin reaction of Cypridina to emit blue light. The luminescence stimulation with major organic cofactors was examined using the crude extracts of Cypridina specimens, and we found that the addition of coenzyme A (CoA) to the crude extracts significantly stimulated luminescence intensity. Further, the light-emitting source in the crude extracts stimulated with CoA was identified as Cypridina luciferyl sulfate, and we demonstrated that CoA could act as a sulfate acceptor from Cypridina luciferyl sulfate. In addition, the sulfate group of Cypridina luciferyl sulfate was also transferred to adenosine 5′-monophosphate (5′-AMP) and adenosine 3′-monophosphate (3′-AMP) by a sulfotransferase. The sulfated products corresponding to CoA, 5′-AMP, and 3′-AMP were identified using mass spectrometry. This is the first report that CoA can act as a sulfate acceptor in a sulfotransferase reaction. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
luciferyl sulfate |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
sulfotransferase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PAP |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
luciferase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
coenzyme A |
| 書誌情報 |
en : Photochemistry and Photobiology
巻 95,
号 6,
p. 1376-1386,
発行日 2019-06-23
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
17511097 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00773103 |
| 出版者 |
|
|
出版者 |
American Society of Photobiology |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Wiley |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is the peer reviewed version of the following article: Nakamura, M., Matsuda, K., Nakamura, M., Yamashita, K., Suzuki, T. and Inouye, S. (2019), Enzymatic Conversion of Cypridina Luciferyl Sulfate to Cypridina Luciferin with Coenzyme A as a Sulfate Acceptor in Cypridina (Vargula) hilgendorfii. Photochem Photobiol, 95: 1376-1386., which has been published in final form at https://doi.org/10.1111/php.13137. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| EID |
|
|
識別子 |
352757 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
php_95_6_1376.pdf (5.23 MB)
