| Item type |
文献 / Documents(1) |
| 公開日 |
2022-09-06 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.jnutbio.2022.109017 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.jnutbio.2022.109017 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Sulforaphane induces lipophagy through the activation of AMPK-mTOR-ULK1 pathway signaling in adipocytes |
|
言語 |
en |
| 著者 |
増田, 真志
ヨシダ シミズ, リサ
モリ, ユウキ
大西, 康太
アダチ, ユウイチロウ
サカイ, マイコ
カブトヤ, セリナ
大南, 博和
(山中)奥村, 仙示
山本, 浩範
ミヤザキ, マコト
竹谷, 豊
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Lipophagy, a form of selective autophagy, degrades lipid droplet (LD) in adipose tissue and the liver. The chemotherapeutic isothiocyanate sulforaphane (SFN) contributes to lipolysis through the activation of hormone-sensitive lipase and the browning of white adipocytes. However, the details concerning the regulation of lipolysis in adipocytes by SFN-mediated autophagy remain unclear. In this study, we investigated the effects of SFN on autophagy in the epididymal fat of mice fed a high-fat diet (HFD) or control-fat diet (CFD) and on the molecular mechanisms of autophagy in differentiated 3T3-L1 cells. Western blotting revealed that the protein expression of lipidated LC3 (LC3-II), an autophagic substrate, was induced after 3T3-L1 adipocytes treatment with SFN. In addition, SFN increased the LC3-II protein expression in the epididymal fat of mice fed an HFD. Immunofluorescence showed that the SFN-induced LC3 expression was co-localized with LDs in 3T3-L1 adipocytes and with perilipin, the most abundant adipocyte-specific protein, in adipocytes of mice fed an HFD. Next, we confirmed that SFN activates autophagy flux in differentiated 3T3-L1 cells using the mCherry-EGFP-LC3 and GFP-LC3-RFP-LC3ΔG probe. Furthermore, we examined the induction mechanisms of autophagy by SFN in 3T3-L1 adipocytes using western blotting. ATG5 knockdown partially blocked the SFN-induced release of fatty acids from LDs in mature 3T3-L1 adipocytes. SFN time-dependently elicited the phosphorylation of AMPK, the dephosphorylation of mTOR, and the phosphorylation of ULK1 in differentiated 3T3-L1 cells. Taken together, these results suggest that SFN may provoke lipophagy through AMPK-mTOR-ULK1 pathway signaling, resulting in partial lipolysis of adipocytes. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Adipose |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Obesity |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Autophagy |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cell biology |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Lipid droplets |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Dietary factors |
| 書誌情報 |
en : The Journal of Nutritional Biochemistry
巻 106,
p. 109017,
発行日 2022-04-21
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09552863 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10748929 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11533735 |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
391705 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jnutbio_106_109017.pdf (1.63 MB)
