Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol

Chen, Shuhai; 池本, 哲也; イケモト, テツヤ; Ikemoto, Tetsuya; 徳永, 卓哉; トクナガ, タクヤ; Tokunaga, Takuya; 沖川, 昌平; オキカワ, ショウヘイ; Okikawa, Shouhei; 宮崎, 克己; ミヤザキ, カツキ; Miyazaki, Katsuki; 山田, 眞一郎; ヤマダ, シンイチロウ; Yamada, Shin-ichiro; 齋藤, 裕; サイトウ, ユウ; Saitou, Yu; 森根, 裕二; モリネ, ユウジ; Morine, Yuji; 島田, 光生; シマダ, ミツオ; Shimada, Mitsuo

doi:https://doi.org/10.1177/09636897221093312

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Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol

https://tokushima-u.repo.nii.ac.jp/records/2010249

名前 / ファイル	ライセンス	アクション
ct_31.pdf (937 KB)

Item type

文献 / Documents(1)

公開日

2022-09-09

アクセス権

open access

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資源タイプ識別子

http://purl.org/coar/resource_type/c_6501

資源タイプ

journal article

出版社版DOI

関連名称

10.1177/09636897221093312

出版タイプ

VoR

出版タイプResource

http://purl.org/coar/version/c_970fb48d4fbd8a85

タイトル

Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol

著者

Chen, Shuhai
池本, 哲也

WEKO 265
徳島大学教育研究者総覧 157341/profile-ja.html
e-Rad_Researcher 20398019

ja	池本, 哲也 ISNI
ja-Kana	イケモト, テツヤ
en	Ikemoto, Tetsuya

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徳永, 卓哉

WEKO 621
徳島大学教育研究者総覧 261398/profile-ja.html
e-Rad_Researcher 30448328

ja	徳永, 卓哉 ISNI
ja-Kana	トクナガ, タクヤ
en	Tokunaga, Takuya

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沖川, 昌平

ja	沖川, 昌平
ja-Kana	オキカワ, ショウヘイ
en	Okikawa, Shouhei

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宮崎, 克己

WEKO 1391
徳島大学教育研究者総覧 361217/profile-ja.html
e-Rad_Researcher 30846126

ja	宮崎, 克己
ja-Kana	ミヤザキ, カツキ
en	Miyazaki, Katsuki

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山田, 眞一郎

WEKO 792
徳島大学教育研究者総覧 294064/profile-ja.html
e-Rad_Researcher 30579884

ja	山田, 眞一郎 ISNI
ja-Kana	ヤマダ, シンイチロウ
en	Yamada, Shin-ichiro

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齋藤, 裕

WEKO 702
徳島大学教育研究者総覧 277637/profile-ja.html
e-Rad_Researcher 50548675

ja	齋藤, 裕 ISNI
ja-Kana	サイトウ, ユウ
en	Saitou, Yu

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森根, 裕二

WEKO 193
徳島大学教育研究者総覧 121751/profile-ja.html
e-Rad_Researcher 60398021

ja	森根, 裕二 ISNI
ja-Kana	モリネ, ユウジ
en	Morine, Yuji

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島田, 光生

WEKO 1253
徳島大学教育研究者総覧 82712/profile-ja.html
e-Rad_Researcher 10216070

ja	島田, 光生 ISNI
ja-Kana	シマダ, ミツオ
en	Shimada, Mitsuo

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内容記述

Peripheral nerve injury (PNI) is a relatively frequent type of trauma that results in the suffering of many patients worldwide every year. Schwann cells (SCs) are expected to be applied in cell therapy because of their ability to promote peripheral nerve regeneration. However, the lack of clinically renewable sources of SCs hinders the application of SC-based therapies. Adipose-derived stem cells (ADSCs) have generated great interest in recent years because of their multipotency and ease of harvest, and they have already been verified to differentiate into Schwann-like cells (SLCs) in vitro. However, the efficiency of differentiation and the functions of SLCs remain unsatisfactory. We newly generated three-dimensional (3D) SLC spheroids from ADSCs using a modified protocol with human recombinant peptide (RCP) petaloid μ-piece. Morphological analysis, gene expression analysis by qRT-PCR, ELISA measurement of the secretion capabilities of neurotrophic factors, and neurite formation assay were performed to evaluate the functions of these 3D SLCs in vitro. Motor function recovery was measured in a sciatic nerve injury mouse model to analyze the nerve regeneration-promoting effect of 3D SLCs in vivo. The differentiation efficiency and the secretion of neurotrophic factors were enhanced in 3D SLCs compared with conventional SLCs. 3D SLCs could more effectively promote neurite growth and longer neurite extension in a neuron-like SH-SY5Y model. Additionally, 3D SLCs had a better therapeutic effect on nerve regeneration after transplantation into the sciatic nerve injury mouse model. These findings demonstrated that the potential of ADSC-derived SLCs to promote nerve regeneration could be significantly increased using our modified differentiation protocol and by assembling cells into a 3D sphere conformation. Therefore, these cells have great potential and can be used in the clinical treatment of PNI.

キーワード

主題

Schwann-like cells

キーワード

主題

adipose-derived stem cells

キーワード

主題

three-dimensional model

キーワード

主題

nerve regeneration

書誌情報

en : Cell Transplantation

巻 31, 発行日 2022-04-25

収録物ID

収録物識別子タイプ

ISSN

収録物識別子

09636897

収録物ID

収録物識別子タイプ

ISSN

収録物識別子

15553892

収録物ID

収録物識別子タイプ

NCID

収録物識別子

AA1084767X

収録物ID

収録物識別子タイプ

NCID

収録物識別子

AA11523185

出版者

SAGE Publications

権利情報

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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386689

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eng

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Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol

× Chen, Shuhai

× 池本, 哲也

× 徳永, 卓哉

× 沖川, 昌平

× 宮崎, 克己

× 山田, 眞一郎

× 齋藤, 裕

× 森根, 裕二

× 島田, 光生

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