| Item type |
文献 / Documents(1) |
| 公開日 |
2022-09-20 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.3389/fncel.2020.598678 |
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関連名称 |
10.3389/fncel.2020.598678 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Chronic Tear Deficiency Sensitizes Transient Receptor Potential Vanilloid 1-Mediated Responses in Corneal Sensory Nerves |
| タイトル別表記 |
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その他のタイトル |
Corneal Nociceptors and Tear Deficiency |
| 著者 |
マスオカ, タカヨシ
ヤマシタ, ユカ
ナカノ, カツヤ
武智, 研志
新村, 貴博
タワ, マサシ
He, Qiang
石澤, 啓介
イシバシ, タカハル
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| 抄録 |
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内容記述 |
Chronic tear deficiency enhances the excitability of corneal cold-sensitive nerves that detect ocular dryness, which can lead to discomfort in patients with dry eye disease (DED). However, changes in corneal nerve excitations through the polymodal nociceptor “transient receptor potential vanilloid 1” (TRPV1) and the potential link between this receptor and symptoms of DED remain unclear. In this study, we examined the firing properties of corneal cold-sensitive nerves expressing TRPV1 and possible contributions of chronic tear deficiency to corneal nerve excitability by TRPV1 activation. The bilateral excision of lacrimal glands in guinea pigs decreased the tear volume and increased the frequency of spontaneous eyeblinks 1–4 weeks after surgery. An analysis of the firing properties of the cold-sensitive nerves was performed by single-unit recordings of corneal preparations 4 weeks after surgery in both the sham-operated and gland-excised groups. Perfusion of the TRPV1 agonist, capsaicin (1 μM), transiently increased the firing frequency in approximately 46–48% of the cold-sensitive nerves characterized by low-background activity and high threshold (LB-HT) cold thermoreceptors in both groups. Gland excision significantly decreased the latency of capsaicin-induced firing in cold-sensitive nerves; however, its magnitude was unchanged. Calcium imaging of cultured trigeminal ganglion neurons from both groups showed that intracellular calcium elevation of corneal neurons induced by a low concentration of capsaicin (0.03 μM) was significantly larger in the gland excision group, regardless of responsiveness to cold. An immunohistochemical study of the trigeminal ganglion revealed that gland excision significantly increased the proportion of corneal neurons enclosed by glial fibrillary acidic protein (GFAP)-immunopositive satellite glial cells. Topical application of the TRPV1 antagonist, A784168 (30 μM), on the ocular surface attenuated eye-blink frequency after gland excision. Furthermore, gland excision enhanced blink behavior induced by a low concentration of capsaicin (0.1 μM). These results suggest that chronic tear deficiency sensitizes the TRPV1-mediated response in the corneal LB-HT cold thermoreceptors and cold-insensitive polymodal nociceptors, which may be linked to dry eye discomfort and hyperalgesia resulting from nociceptive stimuli in aqueous-deficient dry eyes. |
| キーワード |
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|
主題 |
cornea |
| キーワード |
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|
主題 |
transient receptor potential vanilloid 1 (TRPV1) |
| キーワード |
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|
主題 |
trigeminal ganglion |
| キーワード |
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|
主題 |
lacrimal gland excision |
| キーワード |
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|
主題 |
cold-sensitive nerve |
| 書誌情報 |
en : Frontiers in Cellular Neuroscience
巻 14,
p. 598678,
発行日 2020-12-23
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
16625102 |
| 出版者 |
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|
出版者 |
Frontiers Media SA |
| 権利情報 |
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|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| EID |
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|
識別子 |
390675 |
| 言語 |
|
|
言語 |
eng |
fncel_14_598678.pdf (3.59 MB)
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