| Item type |
文献 / Documents(1) |
| 公開日 |
2022-09-22 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
中国産アオゴカイPerinereis aibuhitensisと徳島産イトメTylorrhynchus heterochaetusの血液に浮遊する巨大ヘモグロビンのグロビン鎖の一次構造比較 |
| タイトル別表記 |
|
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その他のタイトル |
Comparative study on the primary structure of globin chains of the extracellular giant hemoglobins from the polychaete Perinereis aibuhitensis and Tylorrhynchus heterochaetus |
| タイトル別表記 |
|
|
その他のタイトル |
多毛類巨大ヘモグロビンの一次構造比較 |
| 著者 |
山中, 瑞恵
脇, 加奈子
尾崎, 絵理香
金井, 拓
金井, 悠一郎
中辻, 崇
谷川, 和代
橋本, 美城子
森, 由美子
倉林, 陽子
岸本, 慎哉
水本, 浩太
渡部, 稔
佐藤, 征弥
真壁, 和裕 W.
大橋, 眞
後藤, 壽夫
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| 抄録 |
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内容記述 |
Amino acid sequences of four types of globin chains a, A, b, and B of the giant Hb from the polychaete Perinereis aibuhitensis, which were collected at the delta of the Yangtze River in the People's Republic of China, were determined by the combination of Edman degradation and RT-PCR method. The primary structures of Perinereis globin chains are compared with those of globins isolated from the polychaete Tylorrhynchus heterochaetus which was collected at the delta of the River Yoshinogawa in Tokushima City. Perinereis globin chains appeared to have 140 amino acid residues for the chain a, 144 for A, 147 for b and B, respectively. These sequences were completely in agreement with the partial cDNA sequences by RT-PCR which covered 119～137 amino acid residues. Some heterogeneities were found for the chain A by protein sequencing. On the other hand, RT-PCR revealed a few polymorphisms in the sequences of chains a, A and b. According to the phylogenetic tree constructed by UPGMA method, Perinereis globin chains were appeared to be separated into two strains A and B at the time later than the separation of vertebrate Mb and Hb but earlier than the separation of the alpha and beta chains of vertebrate Hb. Perinereis globins b and B appeared to be separated from globin A after the time when the trimeric globin chains A, b, and B had been separated clearly from the derivative of globin a according to the phylogenetic tree constructed by NJ method. The homologies between the corresponding globin chains of Perinereis and Tylorrhynchus Hbs were estimated to be within the range of 48～84%. |
| キーワード |
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|
主題 |
giant hemoglobin |
| キーワード |
|
|
主題 |
annelid |
| キーワード |
|
|
主題 |
polychaete |
| キーワード |
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|
主題 |
phylogenetic tree |
| キーワード |
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|
主題 |
supramolecule |
| 書誌情報 |
ja : 自然科学研究
en : Natural Science Research
巻 19,
p. 63-92,
発行日 2005-12-24
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09146385 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10065859 |
| 出版者 |
|
|
出版者 |
徳島大学総合科学部 |
| EID |
|
|
識別子 |
141941 |
| 言語 |
|
|
言語 |
jpn |
nsr_19_63.pdf (3.72 MB)
