| Item type |
文献 / Documents(1) |
| 公開日 |
2022-09-27 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3389/fimmu.2021.634923 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3389/fimmu.2021.634923 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Respiratory FimA-Specific Secretory IgA Antibodies Upregulated by DC-Targeting Nasal Double DNA Adjuvant Are Essential for Elimination of Porphyromonas gingivalis |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Porphyromonas gingivalis Clearance by SIgA |
|
言語 |
en |
| 著者 |
片岡, 宏介
カワバタ, シゲタダ
コヤナギ, カヨ
ハシモト, ヨシヤ
ミヤケ, タツロウ
フジハシ, コウタロウ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Our previous studies showed that a combination of a DNA plasmid encoding Flt3 ligand (pFL) and CpG oligodeoxynucleotides 1826 (CpG ODN) (FL/CpG) as a nasal adjuvant provoked antigen-specific immune responses. In this study, we investigated the efficacy of a nasal vaccine consisting of FimA as the structural subunit of Porphyromonas gingivalis (P. gingivalis) fimbriae and FL/CpG for the induction of FimA-specific antibody (Ab) responses and their protective roles against nasal and lung infection by P. gingivalis, a keystone pathogen in the etiology of periodontal disease. C57BL/6 mice were nasally immunized with recombinant FimA (rFimA) plus FL/CpG three times at weekly intervals. As a control, mice were given nasal rFimA alone. Nasal washes (NWs) and bronchoalveolar lavage fluid (BALF) of mice given nasal rFimA plus FL/CpG resulted in increased levels of rFimA-specific secretory IgA (SIgA) and IgG Ab responses when compared with those in controls. Significantly increased numbers of CD8- or CD11b-expressing mature-type dendritic cells (DCs) were detected in the respiratory inductive and effector tissues of mice given rFimA plus FL/CpG. Additionally, significantly upregulated Th1/Th2-type cytokine responses by rFimA-stimulated CD4+ T cells were noted in the respiratory effector tissues. When mice were challenged with live P. gingivalis via the nasal route, mice immunized nasally with rFimA plus FL/CpG inhibited P. gingivalis colonization in the nasal cavities and lungs. In contrast, controls failed to show protection. Of interest, when IgA-deficient mice given nasal rFimA plus FL/CpG were challenged with nasal P. gingivalis, the inhibition of bacterial colonization in the respiratory tracts was not seen. Taken together, these results show that nasal FL/CpG effectively enhanced DCs and provided balanced Th1- and Th2-type cytokine response-mediated rFimA-specific IgA protective immunity in the respiratory tract against P. gingivalis. A nasal administration with rFimA and FL/CpG could be a candidate for potent mucosal vaccines for the elimination of inhaled P. gingivalis in periodontal patients. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nasal vaccine |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
recombinant FimA (rFimA) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
dendritic cells (DCs) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
double DNA adjuvant |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
mucosal IgA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Porphyromonas gingivalis |
| 書誌情報 |
en : Frontiers in Immunology
巻 12,
p. 634923,
発行日 2021-02-25
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
16643224 |
| 出版者 |
|
|
出版者 |
Frontiers Media S.A. |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| EID |
|
|
識別子 |
374190 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
fimmu_12_634923.pdf (639 KB)
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