| Item type |
文献 / Documents(1) |
| 公開日 |
2022-09-27 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
アオゴカイ巨大ヘモグロビンの再構成 |
| タイトル別表記 |
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その他のタイトル |
Reassociation of annelid giant hemoglobin from the polychaete Perinereis aibuhitensis |
| 著者 |
金井, 拓
佐野, 敏也
板東, 孝枝
金山, 明宏
中西, 優子
脇, 加奈子
村上, 聡
倉富, 久子
徳永, 和歌子
澁谷, 明宏
山下, 智子
佐藤, 高則
後藤, 壽夫
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| 抄録 |
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内容記述 |
Annelid extracellular hemoglobin (Hb) is a supramolecule with molecular mass of ～3,500kDa. The giant Hb consists of 12 subassemblies (globin dodecamers, D) and 18 homodimeric linkers (L) of non-globin chain. The globin dodecamer and linker were isolated from the polychaete Perinereis aibuhittensis Hb separately. Subsequently, these two components were mixed in the presence of 1M urea at a neutral pH to reform a whole molecule of Hb. At first L was refined by reverse phase chromatography in organic solvent. On the other hand, Perinereis Hb was incubated in 4M urea solution at 4°C for 5 min, and applied to two amphoteric ion-exchange resin column to remove L stick to the resin, and to isolate only D. The eluate was condensed and subjected to gel filtration. As a result, an ingredient of molecule mass ～210 kDa, that is D, was provided in high yield. When D and L were mixed in the molar ratio of approximately 1:1 in 50mM phosphate buffer (pH 7.2) in the presence of 1 M urea at room temperature, most of the proteins met to natural Hb size again within about 20 hours. Furthermore, similar experiments were performed in 50 mM Tris-HCl buffer (pH 7.2) containing 1 M urea in the presence of 1 mM CaCl2 or 1mM EDTA. It was observed that the reassociation was affected substantially by the presence of Ca2+. In conclusion, the homodimeric linkers have the key role to form the gigantic Hb. |
| キーワード |
|
|
主題 |
giant Hemoglobin |
| キーワード |
|
|
主題 |
annelid |
| キーワード |
|
|
主題 |
polychaete |
| キーワード |
|
|
主題 |
supramolecule |
| 書誌情報 |
ja : 自然科学研究
en : Natural Science Research
巻 21,
p. 19-32,
発行日 2007-12-21
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09146385 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10065859 |
| 出版者 |
|
|
出版者 |
徳島大学総合科学部 |
| EID |
|
|
識別子 |
165650 |
| 言語 |
|
|
言語 |
jpn |
nsr_21_19.pdf (2.03 MB)
