| Item type |
文献 / Documents(1) |
| 公開日 |
2022-12-09 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1038/s41556-022-00903-1 |
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言語 |
ja |
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|
関連名称 |
10.1038/s41556-022-00903-1 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Cell cycle-specific phase separation regulated by protein charge blockiness |
|
言語 |
en |
| 著者 |
ヤマザキ, ヒロヤ
タカギ, マサトシ
小迫, 英尊
ヒラノ, タツヤ
ヨシムラ, シゲ
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Dynamic morphological changes of intracellular organelles are often regulated by protein phosphorylation or dephosphorylation1-6. Phosphorylation modulates stereospecific interactions among structured proteins, but how it controls molecular interactions among unstructured proteins and regulates their macroscopic behaviours remains unknown. Here we determined the cell cycle-specific behaviour of Ki-67, which localizes to the nucleoli during interphase and relocates to the chromosome periphery during mitosis. Mitotic hyperphosphorylation of disordered repeat domains of Ki-67 generates alternating charge blocks in these domains and increases their propensity for liquid–liquid phase separation (LLPS). A phosphomimetic sequence and the sequences with enhanced charge blockiness underwent strong LLPS in vitro and induced chromosome periphery formation in vivo. Conversely, mitotic hyperphosphorylation of NPM1 diminished a charge block and suppressed LLPS, resulting in nucleolar dissolution. Cell cycle-specific phase separation can be modulated via phosphorylation by enhancing or reducing the charge blockiness of disordered regions, rather than by attaching phosphate groups to specific sites. |
|
言語 |
en |
| 書誌情報 |
en : Nature Cell Biology
巻 24,
号 5,
p. 625-632,
発行日 2022-05-05
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
14657392 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
14764679 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11338922 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11869962 |
| 出版者 |
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|
出版者 |
Springer Nature |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
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|
識別子 |
390920 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ncb_24_5_625.pdf (13.2 MB)
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