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Lrriq1 is an essential factor for fertility by suppressing apoptosis
https://tokushima-u.repo.nii.ac.jp/records/2010389
https://tokushima-u.repo.nii.ac.jp/records/20103897c8dfb3d-ba8a-46f2-83c3-f6733faca190
| 名前 / ファイル | ライセンス | アクション |
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jarg_39_11_2647.pdf (2.75 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||
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| 公開日 | 2022-10-18 | |||||||||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||||||||
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| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s10815-022-02623-9 | |||||||||||||||||||||||||||||
| 関連名称 | 10.1007/s10815-022-02623-9 | |||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||||||||||
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| タイトル | Lrriq1 is an essential factor for fertility by suppressing apoptosis | |||||||||||||||||||||||||||||
| 著者 |
フクトミ, マユ
× フクトミ, マユ
× ウエドノ, チハル
× フジイ, アキ
× 佐藤, 陽一
WEKO
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| 内容記述 | Purpose Leucine-rich repeats and IQ motif containing 1 (LRRIQ1) gene is reportedly associated with plasma inhibin B levels. However, the function of LRRIQ1 remains unknown. In this study, we generated Lrriq1 knockout mice (Lrriq1-/- mice) and examined the effects of LRRIQ1 on inhibin B and fertility. Methods Lrriq1-/- mice were generated using CRISPR/Cas9 genome editing technology. The expression of Inhibin B was examined by Western blotting using a protein extracted from the testis of a 3-month-old male mouse. Mating experiments were conducted using 7-week-old Lrriq1-/-mice and wild-type (WT) mice to examine fertility. Sperm concentration and sperm motility were measured using 3-month-old male mice. Results Expression analysis of inhibin B revealed that Lrriq1-/- mice exhibited reduced mRNA and protein levels of inhibin alpha (Inha), which constitutes the α subunit. In the mating experiment, the litter size of Lrriq1-/- male mice was 4.3 ± 2.9, which was significantly lower than that of WT male mice (8.3 ± 1.3) (p < 0.001). No difference in sperm count was observed between Lrriq1-/- and WT male mice; however, sperm motility (%) was significantly reduced in Lrriq1-/- mice (48.4 ± 4.9) when compared with WT mice (70.2 ± 4.7) (p < 0.001). Based on TUNEL staining, the testes and epididymal sperm of Lrriq1-/- mice showed high numbers of apoptosis-positive cells. Conclusion Lrriq1 knockout reduced sperm motility and litter size by inducing apoptosis of testicular germ cells and epididymal sperm. |
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| 主題 | Lrriq1 | |||||||||||||||||||||||||||||
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| 主題 | Inhibin B | |||||||||||||||||||||||||||||
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| 主題 | Fertility | |||||||||||||||||||||||||||||
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| 主題 | Sperm motility | |||||||||||||||||||||||||||||
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| 主題 | Apoptosis | |||||||||||||||||||||||||||||
| 書誌情報 |
en : Journal of Assisted Reproduction and Genetics 巻 39, 号 11, p. 2647-2657, 発行日 2022-09-21 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 10580468 | |||||||||||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 15737330 | |||||||||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||||||
| 収録物識別子 | AA10830983 | |||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||||||||||||
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| 値 | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use (https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms), but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10815-022-02623-9 | |||||||||||||||||||||||||||||
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| 識別子 | 392232 | |||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||
