| Item type |
文献 / Documents(1) |
| 公開日 |
2023-01-26 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1186/s13568-021-01193-w |
|
|
言語 |
ja |
|
|
関連名称 |
10.1186/s13568-021-01193-w |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus |
|
言語 |
en |
| 著者 |
Boontawon, Tatpong
ナカザワ, タケヒト
イノウエ, チカコ
刑部, 敬史
カワウチ, モリユキ
サカモト, マサヒロ
ホンダ, ヨウイチ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Pleurotus ostreatus is one of the most commercially produced edible mushrooms worldwide. Improved cultivated strains with more useful traits have been obtained using classical breeding, which is laborious and time-consuming. Here, we attempted efficient gene mutagenesis using plasmid-based CRISPR/Cas9 as the first step for non-genetically modified (non-GM) P. ostreatus generation. Plasmids harboring expression cassettes of Cas9 and different single guide RNAs targeting fcy1 and pyrG were individually transferred into fungal protoplasts of the PC9 strain, which generated some strains exhibiting resistance to 5-fluorocytosine and 5-fluoroorotic acid, respectively. Genomic PCR followed by sequencing revealed small insertions/deletions or insertion of a fragment from the plasmid at the target site in some of the drug-resistant strains. The results demonstrated efficient CRISPR/Cas9-assisted genome editing in P. ostreatus, which could contribute to the molecular breeding of non-GM cultivated strains in the future. Furthermore, a mutation in fcy1 via homology-directed repair using this CRISPR/Cas9 system was also efficiently introduced, which could be applied not only for precise gene disruption, but also for insertions leading to heterologous gene expression in this fungus. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Agaricomycete |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mushroom |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
fcy1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
pyrG |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Genome editing |
| 書誌情報 |
en : AMB Express
巻 11,
p. 30,
発行日 2021-02-20
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
21910855 |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
|
|
識別子 |
391866 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ambe_11_30.pdf (1.82 MB)
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