| Item type |
文献 / Documents(1) |
| 公開日 |
2023-08-03 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1111/cas.13448 |
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|
関連名称 |
10.1111/cas.13448 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
ASC amino acid transporter 2, defined by enzyme-mediated activation of radical sources, enhances malignancy of GD2-positive small-cell lung cancer |
| 著者 |
エサキ, ノブトシ
オオカワ, ユウキ
橋本, 登
ツダ, ユウスケ
オオウミ, ユウスケ
Bhuiyan, Robiul H.
コタニ, ノリヒロ
ホンケ, コウイチ
エノモト, アツシ
タカハシ, マサヒデ
フルカワ, ケイコ
フルカワ, コウイチ
|
| 抄録 |
|
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内容記述 |
Ganglioside GD2 is specifically expressed in small-cell lung cancer (SCLC) cells, leading to enhancement of malignant phenotypes, such as cell proliferation and migration. However, how GD2 promotes malignant phenotypes in SCLC cells is not well known. In this study, to reveal the mechanisms by which GD2 increases malignant phenotypes in SCLC cells, we used enzyme-mediated activation of radical sources combined with mass spectrometry in GD2+ SCLC cells. Consequently, we identified ASC amino acid transporter 2 (ASCT2), a major glutamine transporter, which coordinately works with GD2. We showed that ASCT2 was highly expressed in glycolipid-enriched microdomain/rafts in GD2+ SCLC cells, and colocalized with GD2 in both proximity ligation assay and immunocytostaining, and bound with GD2 in immunoprecipitation/TLC immunostaining. Malignant phenotypes of GD2+ SCLC cells were enhanced by glutamine uptake, and were suppressed by L-γ-glutamyl-p-nitroanilide, a specific inhibitor of ASCT2, through reduced phosphorylation of p70 S6K1 and S6. These results suggested that ASCT2 enhances glutamine uptake in glycolipid-enriched microdomain/rafts in GD2+ SCLC cells, leading to the enhancement of cell proliferation and migration through increased phosphorylation of the mTOR complex 1 signaling axis. |
| キーワード |
|
|
主題 |
ASCT2 |
| キーワード |
|
|
主題 |
GD2 |
| キーワード |
|
|
主題 |
glutamine transporter |
| キーワード |
|
|
主題 |
glycolipid |
| キーワード |
|
|
主題 |
small-cell lung cancer |
| 書誌情報 |
en : Cancer Science
巻 109,
号 1,
p. 141-153,
発行日 2017-11-19
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13497006 |
| 出版者 |
|
|
出版者 |
Japanese Cancer Association |
| 出版者 |
|
|
出版者 |
John Wiley & Sons |
| 権利情報 |
|
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
| EID |
|
|
識別子 |
359054 |
| 言語 |
|
|
言語 |
eng |
cas_109_1_141.pdf (1.15 MB)
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