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Identification of lactate dehydrogenase as a mammalian pyrroloquinoline quinone (PQQ)-binding protein
https://tokushima-u.repo.nii.ac.jp/records/2010562
https://tokushima-u.repo.nii.ac.jp/records/2010562b1211dc6-0ef2-4b76-b2d8-a897941ea3ca
| 名前 / ファイル | ライセンス | アクション |
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srep_6_26723.pdf (2.8 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||||||||||||||
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| 公開日 | 2023-09-06 | |||||||||||||||||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1038/srep26723 | |||||||||||||||||||||||||||||||||||||||||
| 関連名称 | 10.1038/srep26723 | |||||||||||||||||||||||||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||||||||||||||
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| タイトル | Identification of lactate dehydrogenase as a mammalian pyrroloquinoline quinone (PQQ)-binding protein | |||||||||||||||||||||||||||||||||||||||||
| 著者 |
赤川, 貢
× 赤川, 貢
WEKO
1472
× ミネマツ, ケンジ
× シバタ, タカヒロ
× コンドウ, タツヒコ
× イシイ, タケシ
× ウチダ, コウジ
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| 内容記述 | Pyrroloquinoline quinone (PQQ), a redox-active o-quinone, is an important nutrient involved in numerous physiological and biochemical processes in mammals. Despite such beneficial functions, the underlying molecular mechanisms remain to be established. In the present study, using PQQ-immobilized Sepharose beads as a probe, we examined the presence of protein(s) that are capable of binding PQQ in mouse NIH/3T3 fibroblasts and identified five cellular proteins, including l-lactate dehydrogenase (LDH) A chain, as potential mammalian PQQ-binding proteins. In vitro studies using a purified rabbit muscle LDH show that PQQ inhibits the formation of lactate from pyruvate in the presence of NADH (forward reaction), whereas it enhances the conversion of lactate to pyruvate in the presence of NAD+ (reverse reaction). The molecular mechanism underlying PQQ-mediated regulation of LDH activity is attributed to the oxidation of NADH to NAD+ by PQQ. Indeed, the PQQ-bound LDH oxidizes NADH, generating NAD+, and significantly catalyzes the conversion of lactate to pyruvate. Furthermore, PQQ attenuates cellular lactate release and increases intracellular ATP levels in the NIH/3T3 fibroblasts. Our results suggest that PQQ, modulating LDH activity to facilitate pyruvate formation through its redox-cycling activity, may be involved in the enhanced energy production via mitochondrial TCA cycle and oxidative phosphorylation. | |||||||||||||||||||||||||||||||||||||||||
| 書誌情報 |
en : Scientific Reports 巻 6, p. 26723, 発行日 2016-05-27 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||||||||||||||
| 収録物識別子 | 20452322 | |||||||||||||||||||||||||||||||||||||||||
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| 出版者 | Springer Nature | |||||||||||||||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||||||||||||||
| 権利情報 | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | |||||||||||||||||||||||||||||||||||||||||
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| 識別子 | 387640 | |||||||||||||||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||||||||||||||
