| Item type |
文献 / Documents(1) |
| 公開日 |
2022-11-29 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
URI |
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関連識別子 |
https://ar.iiarjournals.org/content/anticanres/33/7/2905.full.pdf |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Construction of an Improved Drug Delivery System Tool with Enhanced Versatility in Cell-targeting |
|
言語 |
en |
| タイトル別表記 |
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その他のタイトル |
An Improved DDS Tool with Versatile Cell-targeting Ability |
|
言語 |
en |
| 著者 |
田端, 厚之
オオクボ, ユキマサ
タムラ, マサト
友安, 俊文
大倉, 一人
長宗, 秀明
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Background/Aim: The aim of this study was to develop an improved drug delivery system (DDS) tool with enhanced versatility in the cell-targeting step using as Z-domain, a modified IgG binding domain of protein A from Staphylococcus aureus, as an IgG adapter domain. Materials and Methods: The chimera protein expression system composed of the Z-domain and chimeric cholesterol-dependent cytolysin mutant named His-Z-CDC(ss)IS was constructed in Escherichia coli. His-Z-CDC(ss)IS was purified by Ni-affinity chromatography, and its abilities for controlled pore formation, membrane binding, IgG binding, and target cell-specific delivery of liposomes carrying medicine were investigated. Results and Discussion: His-Z-CDC(ss)IS purified by Ni-affinity chromatography indicated pore-forming activity only under disulfide bond reducing conditions. His-Z-CDC(ss)IS also demonstrated an ability to bind both IgG and cholesterol-embedded liposomes via its Z-domain and domain 4, respectively. Furthermore, anticarcinoembryonic antigen (CEA) IgG-bound His-Z-CDC(ss)IS indicated effective delivery of liposomes carrying drugs to CEA-expressing cells. Conclusion: His-Z-CDC(ss)IS was revealed to be an improved DDS tool with enhanced versatility in cell targeting. |
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言語 |
en |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Cholesterol-dependent cytolysin |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
drug delivery system |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Z-domain |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
chimera protein |
| 書誌情報 |
en : Anticancer Research
巻 33,
号 7,
p. 2905-2910,
発行日 2013-06-18
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
02507005 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
17917530 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10625860 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12440673 |
| 出版者 |
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出版者 |
The International Institute of Anticancer Research |
|
言語 |
en |
| EID |
|
|
識別子 |
280368 |
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識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
anticanres_33_7_2905.pdf (152 KB)
